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| 落叶松杂种与亲本ISSR鉴别技术 | |
| 引用本文： | 张含国,张磊,朱航勇,徐悦丽,姚宇.落叶松杂种与亲本ISSR鉴别技术[J].东北林业大学学报,2011,39(7):1-4. |
| 作者姓名： | 张含国 张磊 朱航勇 徐悦丽 姚宇 |
| 作者单位： | 1. 林木遗传育种国家重点实验室(东北林业大学),哈尔滨,150040 2. 七台河市园林管理局 3. 林木遗传育种国家重点实验室(东北林业大学) |
| 基金项目： | 国家科技基础性工作专项，黑龙江省科技攻关项目 |
| 摘 要： | 以兴安落叶松（Larix gmelini）、长白落叶松（Larix olgensis）和日本落叶松（Larix Kaempferi）及其杂种子代当年生嫩叶为研究材料,采用ISSR分子标记技术对落叶松亲本与杂种进行了鉴别.结果表明:用ISSR16和ISSR19号引物扩增的特异条带能够分别鉴别兴安落叶松×长白落叶松杂交种和... |
| 关 键 词： | 落叶松 杂交种 亲本 ISSR 鉴别 |
ISSR Identification Technology of Hybrid Larch Progenies and Their Parents |
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| Zhang Hanguo,Zhang Lei,Zhu Hangyong,Xu Yueli,Yao Yu.ISSR Identification Technology of Hybrid Larch Progenies and Their Parents[J].Journal of Northeast Forestry University,2011,39(7):1-4. | |
| Authors: | Zhang Hanguo Zhang Lei Zhu Hangyong Xu Yueli Yao Yu |
| Institution: | (State Key Laboratory of Forest Tree Genetic Improvement(Northeast Forestry University)) |
| Abstract: | A study was conducted to identify hybrid larch progenies and their parents by ISSR markers using the early needles of Larix gmelini,L.olgensis,L.Kaempferi and their hybrid progenies as research materials.Result showed that the hybrids of L.gmelini × L.olgensis and L.kaempferi × L.olgensis could be identified with specific bands amplified by primer ISSR16 and ISSR19 respectively.When one parent was the same among different families,the possibility of the other parent could be calculated by amplified bands.Reciprocal cross families could be identified in terms of the appearance rate of target bands more than 80 percent.All the families could be classified into four kinds of hybrids at a genetic distance of 0.4,namely L.kaempferi × L.gmelini,L.kaempferi × L.olgensis,L.gmelini × L.olgensis and L.gmelini × L.kaempferi,which was consistent with the result of molecular markers. |
| Keywords: | Larch Hybrids Parents ISSR Identification |
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