| 应用RSAP、SRAP和SSR分析苎麻种质亲缘关系 |
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| 引用本文： | 邹自征,陈建华,栾明宝,郭劲霞,王超,王晓飞,许英,孙志民.应用RSAP、SRAP和SSR分析苎麻种质亲缘关系[J].作物学报,2012,38(5):840-847. |
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| 作者姓名： | 邹自征 陈建华 栾明宝 郭劲霞 王超 王晓飞 许英 孙志民 |
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| 作者单位： | 1中国农业科学院麻类研究所 / 农业部茎纤维生物质与工程微生物重点开放实验室, 湖南长沙 410205；2益阳医学高等专科学校, 湖南益阳413000；3中南大学生物科学与技术学院，湖南长沙 410205 |
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| 基金项目： | 国家自然科学基金项目(30900913);国家科技支撑计划项目(2006BAD13B04-2);中国农业科学院科技经费项目资助 |
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| 摘 要： | 分别采用RSAP、SRAP和SSR 3种分子标记和田间性状对16份苎麻种质进行亲缘关系聚类，结果表明, 每对引物扩增出的多态性位点，SRAP标记最多，RSAP次之，SSR较少；根据SRAP标记和RSAP标记分类的结果都与RSAP+SRAP+SSR联合分类的结果基本一致，相关达到了极显著水平，而与SSR标记分类结果差异较大；分子标记聚类结果与田间性状的聚类结果接近程度, 依次为SRAP+RSAP+SSR>SRAP>RSAP>>SSR。在检测苎麻种质亲缘关系中，SRAP标记效果最优，RSAP标记稍逊，SSR标记最差。RSAP标记能较好地显示苎麻种属间的多态性和亲缘关系。以RSAP、SRAP、SSR标记联合分析，能更好地揭示种质之间的亲缘关系。
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| 关 键 词： | 苎麻 RSAP SRAP SSR 亲缘关系 |
| 收稿时间： | 2011-10-13 |
Genetic Relationship of Ramie Germplasm Revealed by RSAP,SRAP,and SSR Markers |
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| ZOU Zi-Zheng,CHEN Jian-Hua,LUAN Ming-Bao,GUO Jin-Xia,WANG Chao,WANG Xiao-Fei,XU Ying,and SUN Zhi-Min.Genetic Relationship of Ramie Germplasm Revealed by RSAP,SRAP,and SSR Markers[J].Acta Agronomica Sinica,2012,38(5):840-847. |
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| Authors: | ZOU Zi-Zheng CHEN Jian-Hua LUAN Ming-Bao GUO Jin-Xia WANG Chao WANG Xiao-Fei XU Ying and SUN Zhi-Min |
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| Institution: | 1.Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences / Key Laboratory of Stem fiber Biomass and Engineering Microbilogy, Ministry of Agriculture, Changsha 420105, China;2.Yiyang Medical School, Yiyang 413000, China;3.School of Biological Science and Technology, Central South University, Changsha 410205, China |
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| Abstract: | The research on molecular biology in ramie Boehmeria nivea(L.)Gaud.] is not as advanced as that in major crops due to the lagged development of molecular markers.We compared the effectiveness of restriction site amplified polymorphism(RSAP),sequence-related amplified polymorphism(SRAP),and simple sequence repeat(SSR)markers in revealing genetic relationship of 16 entries of ramie germplasm.The SRAP marker detected the most polymorphic loci,followed by the RSAP marker,and SSR marker had the lowest polymorphism.The dendrogram based on SRAP or RSAP data showed high consistency with that based on combined data of SRAP+RSAP+SSR,and their genetic distances were significantly correlated.In contrast,the SSR data constructed a dendrogram with different clusters of the 16 entries.The similarity of clusters between phenotypic traits and molecular markers was sequenced as SRAP+RSAP+SSR>RSAP>SRAP>SSR.The SPAR marker had the highest efficiency to disclose the genetic relationship among ramie germplasm,which was slightly higher than that of the RSAP marker,whereas the SSR showed the lowest efficiency.Thus,RSAP marker was applicable in diversity analysis in ramie,and the joint use of RSAP,SRAP,and SSR markers may enhance the reliability of genetic relationship. |
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| Keywords: | Ramie RSAP SRAP SSR Relationship |
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