| 非生物胁迫诱导的GmMYB的克隆与表达分析 |
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| 引用本文： | 孙霞,刘晋跃,袁晓辉,潘相文,杜维广,任海祥,马永波,Jun ABE,邱丽娟,刘宝辉.非生物胁迫诱导的GmMYB的克隆与表达分析[J].作物学报,2012,38(2):360-368. |
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| 作者姓名： | 孙霞 刘晋跃 袁晓辉 潘相文 杜维广 任海祥 马永波 Jun ABE 邱丽娟 刘宝辉 |
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| 作者单位： | 1中国科学院东北地理与农业生态研究所大豆分子育种实验室 / 中国科学院黑土区农业生态院重点实验室, 黑龙江哈尔滨 150081; 2黑龙江省农业科学院牡丹江分院 / 国家大豆改良中心牡丹江试验站, 黑龙江牡丹江 157041; 3辽宁省农业环境保护监测站, 辽宁沈阳 110034; 4日本北海道大学农学院, 日本札幌 060-8589; 5中国农业科学院作物科学研究所, 北京 100081 |
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| 基金项目： | 中国科学院“百人计划”(KZCX2-YW-BR-11);国家自然科学基金项目(30971813和31101170);黑龙江省杰出青年基金(JC200919);黑龙江省青年基金(QC2011C015);黑龙江省归国基金(09SRS11);教育部留学回国人员科研启动基金项目(Y0SQY11001);所前沿领域项目(2009ZX08009-013B)资助 |
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| 摘 要： | 本研究室根据一段抗逆的EST序列, 从栽培大豆东农42中克隆到4个开放阅读框均是外显子和内含子间隔构成的R2R3-MYB基因, 其中Gm02g01300、Gm03g38040和Gm10g01340与已公布的Willams 82基因组序列完全一致, Gm19g40650第375位的单核苷酸突变导致多肽链第125位的氨基酸发生置换(GAG375→GAC375, E125→D125)。以人工气候箱内模拟非生物胁迫(盐、碱、干旱和低温)处理栽培大豆东农42芽期, 选择适宜时间点, 采用荧光定量PCR技术, 检测R2R3-MYB基因的表达。结果表明, 4个基因的表达水平都存在明显波动, 呈诱导后短暂上调或下调两种表达模式, 但表达时间、强度和趋势存在明显差异; Gm02g01300受干旱诱导明显, Gm03g38040受多种胁迫条件诱导表达强烈, 推测这些基因在大豆非生物胁迫的调控中起到重要作用; 另外, 在子叶与胚间, 单个基因的表达也存在差异; 多种非生物胁迫条件下, 基因的表达不仅存在时空差异, 可能也具有调控模式的差异。
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| 关 键 词： | 非生物胁迫 GmMYB 芽期 表达分析 |
| 收稿时间： | 2011-06-21 |
Cloning and Expression Analysis of GmMYB Genes Induced by Abiotic Stresses |
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| SUN Xia,LIU Jin-Yue,YUAN Xiao-Hui,PAN Xiang-Wen,DU Wei-Guang,REN Hai-Xiang,MA Yong-Bo,Jun ABE,QIU Li-Juan,and LIU Bao-Hui.Cloning and Expression Analysis of GmMYB Genes Induced by Abiotic Stresses[J].Acta Agronomica Sinica,2012,38(2):360-368. |
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| Authors: | SUN Xia LIU Jin-Yue YUAN Xiao-Hui PAN Xiang-Wen DU Wei-Guang REN Hai-Xiang MA Yong-Bo Jun ABE QIU Li-Juan and LIU Bao-Hui |
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| Institution: | 1,1 Laboratory of Soybean Molecular Breeding,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences /Key Laboratory of Mollisols Agroecology,Chinese Academy of Sciences,Harbin 150081,China;2 Mudanjiang Branch of Heilongjiang Academy of Agricultural Sci-ences,Mudanjiang 157041,China;3 Liaoning Agricultural Environmental Protection Monitoring Station,Shenyang 110034,China;4 Research Fac-ulty of Agriculture,Hokkaido University,Sapporo,Hokkaido 060-8589,Japan;5 Institute of Crop Sciences,Chinese Academy of Agricultural Sci-ences,Beijing 100081,China |
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| Abstract: | Response to external environment is the outcome of stress-induced gene expression.In this paper,based on one stress-induced EST sequence,we cloned four R2R3-MYB genes from soybean cultivar Dongnong 42,whose genomic sequences con-sisted of three exons and two introns.Three of them corresponding to Gm02g1300,Gm03g38040,and Gm10g01340 are respectively consistent with the sequences of Williams 82.A mutation at the 375th single nucleotide in the sequence of Gm19g40650 from Dongnong 42 caused a synonymous amino acid substitution(E125-D125).To test the relationship of four MYB genes with stress resis-tance,we treated the seedlings of cultivar Dongnong 42 with abiotic stresses including salt,alkali,drought and low temperature in the artificial climate chamber.Quantitative PCR analysis indicated that all of the four genes were transient down-regulated or up-regulated when subjected to the stresses,but different in the expression time,level and tendency.Gm02g01300 was induced by drought stress while Gm03g38040 was strongly induced by multiple stresses,indicating that they play important roles in responding to external stresses.There were also differences in the expression of individual gene between cotyledons and embryos.These results under a variety of abiotic stress conditions suggest that the four R2R3-MYB genes are different not only in the expression patterns,but also in the regulation modes. |
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| Keywords: | Abiotic stress GmMYB Bud period Expression analysis |
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