| 超级稻花后强、弱势粒灌浆相关蛋白质表达的差异 |
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| 引用本文： | 陈婷婷,谈桂露,褚光,刘立军,杨建昌*.超级稻花后强、弱势粒灌浆相关蛋白质表达的差异[J].作物学报,2012,38(8):1471-1482. |
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| 作者姓名： | 陈婷婷 谈桂露 褚光 刘立军 杨建昌* |
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| 作者单位： | 扬州大学江苏省作物遗传生理重点实验室，江苏扬州225009 |
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| 基金项目： | 国家自然科学基金重大国际合作交流项目(31061140457),国家自然科学基金项目(31071360);江苏省基础研究计划项目(BK2009005);2011年中央级科研院所基本科研业务费专项(农业)(201103003);江苏省高校优势学科建设工程资助项目资助 |
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| 摘 要： | 为进一步揭示水稻强、弱势粒灌浆差异的机理，以2个超级稻品种两优培九(两系杂交籼稻)和淮稻9号(粳稻)为材料，通过双向电泳观察花后强、弱势粒灌浆相关蛋白质表达的差异并对差异蛋白进行质谱分析。结果表明，弱势粒花后3~15 d的灌浆速率和最终粒重均显著低小于强势粒。花后3 d、8 d和15 d弱势粒中蛋白质表达的点少于强势粒，花后25 d则弱势粒多于强势粒。花后强、弱势粒间差异蛋白质表达量在2倍以上的点有类似变化趋势。选择有显著差异的27个蛋白点进行质谱分析，其中14个点的功能得到鉴定。这些蛋白质分别参与籽粒的淀粉合成、蛋白合成、光合作用、能量代谢、环境适应以及细胞信号转导等。说明强、弱势粒间多种灌浆相关蛋白质表达的差异是其灌浆和粒重差异的重要原因。
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| 关 键 词： | 超级稻 强、弱势粒 灌浆相关蛋白质表达 蛋白质功能 籽粒灌浆 |
| 收稿时间： | 2012-01-13 |
Differential Expressions of the Proteins Related to Grain Filling between Superior and Inferior Spikelets of Super Rice after Anthesis |
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| CHEN Ting-Ting,TAN Gui-Lu,CHU Guang,LIU Li-Jun,and YANG Jian-Chang.Differential Expressions of the Proteins Related to Grain Filling between Superior and Inferior Spikelets of Super Rice after Anthesis[J].Acta Agronomica Sinica,2012,38(8):1471-1482. |
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| Authors: | CHEN Ting-Ting TAN Gui-Lu CHU Guang LIU Li-Jun and YANG Jian-Chang |
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| Institution: | Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou 225009, China |
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| Abstract: | Poor grain filling of the later-flowered inferior spikelets(in contrast to the earlier-flowered superior spikelets) is a serious problem in rice production.This problem is more aggravated in new bred super rice cultivars.To better understand the mechanism underlying the poor grain filling of inferior spikelets,we investigated the difference in expressions of the proteins related to grain filling in superior and inferior spikelets of two super rice cultivars,Liangyoupeijiu(indica hybrids) and Huaidao 9(japonica) after anthesis through two-dimensional gel electrophoresis(SDS-PAGE) and the MALDI analysis.Results showed that the grain filling rate at 3-15 d after anthesis(DAA) and the final grain weight of inferior spikelets were much smaller than those of superior spikelets.The spots of protein expressed were fewer in inferior spikelets than in superior spikelets at 3,8,and 15 DAA,and the results were reversed at 25 DAA.The spots of protein with two-fold expressions between superior and inferior spikelets showed a similar changing tendency.Twenty seven protein spots with significant difference in the expression between superior and inferior spikelets were chosen for the MALDI analysis,of which 14 protein spots were identified and their functions were analyzed.These proteins were involved in grain starch synthesis,protein synthesis,photosynthesis,energy metabolism,environmental adaptation and cell signal transduction.The results suggest that expression differences of the proteins related to grain filling account for the variation in grain filling between superior and inferior spikelets of rice. |
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| Keywords: | Super rice Superior/inferior spikelets Expressions of the proteins related to grain filling Protein function Grain filling |
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