| 小麦品种小偃9323抗条锈基因的遗传分析和分子作图 |
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| 引用本文： | 唐明双,马东方,王海鸽,尹军良,侯璐,姚强,井金学.小麦品种小偃9323抗条锈基因的遗传分析和分子作图[J].作物学报,2013,39(5):855-861. |
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| 作者姓名： | 唐明双 马东方 王海鸽 尹军良 侯璐 姚强 井金学 |
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| 作者单位： | 旱区作物逆境生物学国家重点实验室 / 西北农林科技大学植物保护学院，陕西杨凌712100 |
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| 基金项目： | 高等学校学科创新引智计划项目，国家公益性(农业)行业科研专项 |
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| 摘 要： | 小偃9323是小偃6号的同源材料，具有早熟、抗逆性强、适应性广、抗条锈性强等许多优良的生物学特性。为明确其抗条锈性及遗传规律，利用当前流行的中国条锈菌小种CYR32对抗病品种小偃9323与感病品种铭贤169及其杂交后代F1、F2、F3和BC1代进行苗期抗条锈性遗传分析，并对其抗条锈基因进行SSR分子标记。结果表明，小偃9323对CYR32小种具有良好的抗性，由1对隐性基因所控制。利用F2代分离群体，筛选到6个与抗病基因连锁的SSR标记，分别是Xwmc807、Xbarc3、Xwmc684、Xwmc201、Xwmc553和Xwmc179；该抗病基因位于小麦6AL染色体上，其最近的标记为Xwmc201和Xwmc553，遗传距离分别是2.6 cM和3.7 cM。分析表明，该基因不同于已知抗条锈基因，暂被命名为YrXY9323。用YrXY9323两侧遗传距离最近的标记Xwmc201和Xwmc553对42个黄淮麦区主栽小麦品种进行分子检测，结果表明有19%的品种具有与YrXY9323相同的标记位点。本结果对YrXY9323在小麦抗条锈病育种中的应用提供了理论依据。
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| 关 键 词： | 小偃9323 小麦条锈菌 隐性抗病基因 分子标记 |
| 收稿时间： | 2012-08-27 |
Genetic Analysis and Molecular Mapping of Stripe Rust Resistance Gene in Wheat Cultivar Xiaoyan 9323 |
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| TANG Ming-Shuang,MA Dong-Fang,WANG Hai-Ge,YIN Jun-Liang,HOU Lu,YAO Qiang,JING Jin-Xue.Genetic Analysis and Molecular Mapping of Stripe Rust Resistance Gene in Wheat Cultivar Xiaoyan 9323[J].Acta Agronomica Sinica,2013,39(5):855-861. |
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| Authors: | TANG Ming-Shuang MA Dong-Fang WANG Hai-Ge YIN Jun-Liang HOU Lu YAO Qiang JING Jin-Xue |
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| Institution: | State Key Laboratory of Crop Stress Biology for Arid Areas / College of Plant Protection, Northwest A&F University, Yangling 712100, China |
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| Abstract: | Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most widespread and destructive wheat diseases worldwide. Resistance breeding is constantly pursued for decades to tackle the variations of prevalent Pst races. Xiaoyan 9323, the homology material of Xiaoyan 6, has early maturity, strong resistance, wide adaptability, higher resistance to stripe rust, excellent biological characteristics. To identify the resistance gene(s), we crossed Xiaoyan 9323 with susceptible genotype Mingxian 169, and tested F1, F2, F3, and BC1 progenies with Chinese race CYR32 at seedling stage in greenhouse. Then, SSR techniques were used to screen molecular markers linked to the gene conferring resistance to CYR32 in Xiaoyan 9323. The genetic analysis results showed that Xiaoyan 9323 displayed resistance to epidemic yellow rust races in China when tested with five races ofstripe rust and one recessive gene conferring resistance to races CYR32. The mapping population of F2 generation was inoculated with CYR32. Six SSR markers, Xwmc807, Xbarc3, Xwmc684, Xwmc201, Xwmc553,and Xwmc179 were linked to the resistance gene, which were all located on chromosome arm 6AL. The closest flanking makers were Xwmc201 and Xwmc553 with genetic distances of 2.6 cM and 3.7 cM, respectively. According to SSR markers’ chromosome location, the gene was located at chromosome 6AL. Based on chromosomal location, reactions to various pathotypes andpedigree analysis, the gene may be a new one different from the known stripe rust resistance genes, designated as YrXY9323 temporarily. Two flanking SSR markers, Xwmc201 and Xwmc553, were used to test 42 wheat cultivars from Huang-Huai River Valleys wheat region. The results showed that 19% cultivars had the same polymorphic bands as YrXY9323, indicating that the polymorphic SSR markers can be useful in wheat molecular marker selection and breeding. |
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| Keywords: | Xiaoyan 9323 Puccinia striiformis f sp tritici Recessive resistance gene Molecular mapping |
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