| 利用芥蓝×青花菜DH群体构建AFLP连锁图谱 |
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| 引用本文： | 王晓武,娄平,何杭军,杨宝军,张延国,赵建军.利用芥蓝×青花菜DH群体构建AFLP连锁图谱[J].园艺学报,2005,32(1):30-34. |
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| 作者姓名： | 王晓武 娄平 何杭军 杨宝军 张延国 赵建军 |
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| 作者单位： | ( 中国农业科学院蔬菜花卉研究所, 北京100081; 浙江大学农业与生物技术学院园艺系, 杭州310029) |
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| 基金项目： | 国家自然科学基金资助项目(30370981,30471188,30400298)，国家‘863’资助项目(2003AA207120) |
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| 摘 要： | 利用青花菜(Brassica oleracea var. italica) 与芥蓝(Brassica oleracea var. alboglabra) 杂交后代双单倍体(DH) 群体为材料, 通过AFLP技术构建了一个甘蓝类作物较高密度的遗传连锁图谱。该图谱包括9个主要连锁群及2个小连锁群, 总图距801.5 cM, 含337个AFLP标记, 标记间平均距离为3.6 cM,为甘蓝类作物基因定位、比较基因组学及重要经济性状QTL分析提供了一个框架图谱。
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| 关 键 词： | 芥蓝 青花菜 DH群体 AFLP 连锁图谱 |
| 文章编号： | 0513-353X(2005)01-0030-05 |
| 收稿时间： | 2004-3-26 |
| 修稿时间： | 2004年3月26日 |
Construction of an AFLP-based Genetic Linkage Map Using a Doubled-haploid (DH)Population Derived from a Cross between Chinese Kale and Broccoli |
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| Wang Xiaowu,LOU Ping,He Hangjun,YANG Baojun,ZHANG Yanguo,ZHAO Jianjun.Construction of an AFLP-based Genetic Linkage Map Using a Doubled-haploid (DH)Population Derived from a Cross between Chinese Kale and Broccoli[J].Acta Horticulturae Sinica,2005,32(1):30-34. |
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| Authors: | Wang Xiaowu LOU Ping He Hangjun YANG Baojun ZHANG Yanguo ZHAO Jianjun |
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| Institution: | ( Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Department of Horticulture, Zhejiang University, Hangzhou 310029, China) |
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| Abstract: | A high-density genetic linkage map of Brassica oleracea was constructed using AFLP markers based on a Brassica oleracea var. alboglabra, Brassica oleracea var italica F 1-derived doubled-haploid (DH) population with 46 lines. The map consists of 9 major linkage groups and 2 minor groups, which includes 337 AFLP markers, and covers 801.5 cM with an average genetic interval of 3.6 cM. This molecular marker map could be used for genetic mapping and molecular marker assisted breeding of Brassica oleracea. |
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| Keywords: | Chinese kale Broccoli Double haploid AFLP Linkage map |
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