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| 奶牛血清中维生素A和维生素E含量的测定及其与牛乳中体细胞数的相关性 | |
| 引用本文： | 董淑慧,王加启,李发弟,彭华,张养东,赵海燕.奶牛血清中维生素A和维生素E含量的测定及其与牛乳中体细胞数的相关性[J].甘肃农业大学学报,2013,48(1):19-25. |
| 作者姓名： | 董淑慧 王加启 李发弟 彭华 张养东 赵海燕 |
| 作者单位： | 1. 甘肃农业大学动物科学技术学院,甘肃兰州 730070;中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京100193 2. 甘肃农业大学动物科学技术学院,甘肃兰州,730070 3. 中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京100193 |
| 摘 要： | 利用高效液相色谱法测定奶牛血清中维生素A和维生素E的含量,并探讨其与牛乳中体细胞数的相关性.结果表明:奶牛血清中维生素A的加标回收率为93.8%～106.4%,维生素E的加标回收率为91.5%～105.3%,RSD分别为4.7%和4.4%,该方法简单快速准确可靠,可用于奶牛血清样品中维生素A和维生素E含量的测定.维生素E浓度在泌乳第4～8周与体细胞数呈弱负相关性(R=-0.25,P=0.037),在泌乳第10～14周为中等程度负相关(R=-0.41,P=0.037);维生素A与体细胞数不存在相关性. |
| 关 键 词： | 奶牛 血清 维生素A 维生素E 体细胞数 相关性 |
Simultaneous determination method of vitamin A and vitamin E in dairy cow serum by HPLC and relationship with SCC in milk |
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| DONG Shu-hui , WANG Jia-qi , LI Fa-di , PENG Hua , ZHANG Yang-dong , ZHAO Hai-yan.Simultaneous determination method of vitamin A and vitamin E in dairy cow serum by HPLC and relationship with SCC in milk[J].Journal of Gansu Agricultural University,2013,48(1):19-25. | |
| Authors: | DONG Shu-hui WANG Jia-qi LI Fa-di PENG Hua ZHANG Yang-dong ZHAO Hai-yan |
| Institution: | 1.College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;2.State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China) |
| Abstract: | To establish HPLC determination method for the content of vitamin A and vitamin E in serum of dairy cow.The analysis column of this method was C18 column(250 mm×4.6 mm,5 μm),the flow phase was methanol,the flow rate was 1.0 mL/min,and the detection wave length for vitamin A and vitamin E was 325 nm and 292 nm,separately.The recoveries for vitamin A and vitamin E were 93.8%~106.4% and 91.5% ~105.3%,RSD was 4.7% and 4.4%,separately.The method that determination of vitamin A and vitamin E in serum of dairy cow by HPLC was simple,rapid,sensitive and accurate.There was a weak but significant correlation between vitamin E and SCC in week 4~8 of lactation(r=-0.25,P=0.037),and a medium but significant correlation in week 8~10 of lactation(r=-0.41,P=0.037),there were no correlation between vitamin A and SCC. |
| Keywords: | dairy cow serum vitamin A vitamin E somatic cell count correlation |
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