| 稻瘟病菌细菌人工染色体（BAC）基因组文库的构建 |
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| 引用本文： | 周晓罡,朱立煌,翟文学,李进斌,罗朝喜,李成云.稻瘟病菌细菌人工染色体（BAC）基因组文库的构建[J].云南农业大学学报,2002,17(4):331-334. |
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| 作者姓名： | 周晓罡 朱立煌 翟文学 李进斌 罗朝喜 李成云 |
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| 作者单位： | [1]云南省农业科学院生物技术研究所，云南昆明650223 [2]中国科学院遗传研究所，北京100101 |
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| 基金项目： | 云南省自然科学基金重点资助项目 (99C0 0 8Z) |
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| 摘 要： | 稻瘟病菌是一种重要的植物病原菌,同时又是研究植物和病原物之间相互关系的一个重要试验系统。实验通过采用95-23-4a稻瘟病菌株经提取纯化得到该稻瘟病菌的细胞核基因组总DNA,用限制性内切酶部分酶解后,与经末端去磷酸化处理过的pCUGIBAC1质粒载体按一定摩尔比例相互连接,通过转化E.coli DH10B感受态细胞进而通过蓝白斑筛选挑取白色克隆从而构建了95-23-4a稻瘟病菌株的细菌人工染色体（BAC）基因组文库。通过进行酶切检测及Southern杂交分析后确定所构建的BAC文库平均插入片段29.1 kb,该文库覆盖7.4倍基因组,此基因组文库的构建为该菌致病相关基因的克隆奠定了基础。
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| 关 键 词： | 稻瘟病菌 基因组 细菌人工染色体（BAC）文库 |
| 文章编号： | 1004-390X(2002)04-0331-04 |
| 收稿时间： | 2002-5-8 |
The Construction of Bacterial Artificial Chromosom (BAC) Library of Rice Blast Pathogen |
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| ZHOU Xiao gang ,ZHU Li huang ,ZHAI Wen xue ,LI Jin bin ,LUO Chao xi ,LI Cheng yun.The Construction of Bacterial Artificial Chromosom (BAC) Library of Rice Blast Pathogen[J].Journal of Yunnan Agricultural University,2002,17(4):331-334. |
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| Authors: | ZHOU Xiao gang ZHU Li huang ZHAI Wen xue LI Jin bin LUO Chao xi LI Cheng yun |
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| Institution: | ZHOU Xiao gang 1,ZHU Li huang 2,ZHAI Wen xue 2,LI Jin bin 3,LUO Chao xi 3,LI Cheng yun 1 |
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| Abstract: | The fungus Magnaporthe grisea is an important plant pathogen and is a useful model organism for studying various aspects of host-pathogen interactions. The fungus infects a wide range of grasses, including barley and millet, but it is best known as the causal agent of the blast disease of rice (Oryza sativa).The authors have constructed a genomic DNA library using the bacterial artificial chromosome(BAC) with Genomic DNA digested HindⅢ was ligated to the BAC vector ,and the ligation mixture was used to transform the bacterial strain DH10B.A total of over 11904 clones were obtained with the average insert size of about 29.1kb, 7.4×covering haploid genome of the fungus.The BAC library can be used for cloning of pathogenical gene. |
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| Keywords: | Magnaporthe grisea genome bacterial artificial chromosome (BAC) library |
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