Field evaluation of lentil cultivars inoculated with Rhizobium leguminosarum bv. viciae strains for nitrogen fixation using nitrogen-15 isotope dilution |
| |
| Authors: | F Y Hafeez N H Shah K A Malik |
| |
| Institution: | (1) National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Faisalabad, Pakistan e-mail: biofert@nibgel.fsb.erum.com.pk Tel.: +92-41-651475/76/77/78/79, Fax: +92-41-651472, PK;(2) Department of Soil Science, University of Agriculture, Faisalabad, Pakistan, PK |
| |
| Abstract: | A 15N isotope dilution technique was applied to quantify the extent of N2 fixation in lentil (Lens culinaris Medik.) cultivars as influenced by Rhizobium leguminosarum bv. viciae strains in a field experiment in Pakistan. The experiment was conducted on a soil with a very small indigenous rhizobial population
and where N was a limiting factor for crop production. Significant variations in number of nodules, dry weight of nodules,
biomass yield, grain yield, total N yield, proportion of plant N derived from N2 fixation (Pfix) and amount of N derived from the atmosphere (Ndfa) were observed among combined treatments of four rhizobial strains and six lentil varieties. In a field previously labelled
with 15N, to which a basal dose of 75 kg P2O5 ha–1 was applied as single super phosphate, Ndfa ranged from 15 to 24 kg N ha–1 when calculated according to rhizobial strain and from 4 to 38 kg N ha–1 when calculated according to lentil variety. Lc 26 was the most effective strain and fixed 243% more N than the indigenous
population in the uninoculated control. In treatments with the lentil variety PL-406, Ndfa was 38 kg N ha–1, which was 850% higher than with the lentil variety Precoz/F6-20-1×M-85. Generally, the varieties with greater Pfix produced a higher dry matter yield.
Received: 26 May 1999 |
| |
| Keywords: | Nitrogen-15 isotope dilution Legumes Lens culinaris Rhizobium Nitrogen fixation |
| 本文献已被 SpringerLink 等数据库收录！ |
|
