| 西瓜新炭疽病病原菌分子鉴定及其生物学特性初探 |
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| 引用本文： | 黄蔚,崔丽红,谢王超,肖国强.西瓜新炭疽病病原菌分子鉴定及其生物学特性初探[J].中国农学通报,2022,38(1):131-136. |
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| 作者姓名： | 黄蔚 崔丽红 谢王超 肖国强 |
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| 作者单位： | 1.湘西民族职业技术学院,湖南湘西 416000;2.永顺县高坪乡农技和农村合作经济服务站,湖南永顺 416000 |
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| 基金项目： | 2018年湖南省教育厅科学研究项目“湘西地区西瓜炭疽病生理小种鉴定及生物防治技术研究”(18C1706)； |
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| 摘 要： | 探究湘西地区西瓜炭疽病致病种类,为西瓜炭疽病防控提供理论依据.以湘西民族职业技术学院科技园种植的西瓜病叶为研究对象,对该病的病原菌进行分离提纯、形态学观察、柯赫氏法则验证、18S rDNA-ITS序列分析以及生物学特性研究.菌株C913菌落呈圆形,菌丝绒毛状,排列整齐,初为白色,后渐为灰白色.轮状排列分生孢子盘,并有橘...
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| 关 键 词： | 西瓜炭疽病 18S rDNA-ITS 生物学特性 胶孢炭疽菌 菌落直径 |
| 收稿时间： | 2021-01-22 |
Molecular Identification of a New Pathogen of Watermelon Anthracnose and Preliminary Study on Its Biological Characteristics |
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| HUANG Wei,CUI Lihong,XIE Wangchao,XIAO Guoqiang.Molecular Identification of a New Pathogen of Watermelon Anthracnose and Preliminary Study on Its Biological Characteristics[J].Chinese Agricultural Science Bulletin,2022,38(1):131-136. |
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| Authors: | HUANG Wei CUI Lihong XIE Wangchao XIAO Guoqiang |
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| Institution: | 1.Xiangxi Vocational and Technical College for Nationalities, Xiangxi, Hunan 416000;2.Agricultural Technology and Rural Cooperative Economic Service Station, Gaoping Township, Yongshun County, Yongshun, Hunan 416000 |
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| Abstract: | To explore the pathogenic species of watermelon anthracnose in western Hunan, and provide a theoretical basis for watermelon anthracnose prevention and control, we took the diseased leaves of watermelon grown in the Science and Technology Park of Xiangxi Vocational and Technical College for Nationalities as the research objects, isolated and purified the pathogen of the disease, and conducted morphological observation, Koch's rule verification, 18S rDNA-ITS sequence analysis and the biological characteristics analysis. The colony of strain C913 was round, hyphae of villiform and neatly arranged. It was white at first and then grayish white. The conidia discs were arranged in a ring shape, and there were orange-red conidia clusters. The conidia are cylindrical, blunt at both ends, single cell, with a septum in the middle, and the size was (9.8~17.5) µm× (4.5~5.9) µm. The 18S rDNA sequence was 100% similar to the four Colletotrichum gloeosporioides included in GenBank. The hyphae could grow normally when the temperature was 10-37℃, but the growth rate of the hyphae was the fastest at 25℃, and the colony diameter was 78.28 mm. The growth slowed down when the temperature was less than 10℃ or greater than 37℃. The hyphae spores could grow under the condition of pH 5-11. The hyphae grew fastest when the pH was 6 and the colony diameter was the largest, 84.62 mm. The colony diameter with glucose and sucrose as the carbon source medium was significantly higher than that of other treatments, 63.4 mm and 62.2 mm, respectively; while lactose was not conducive to the growth of mycelium (P<0.01), which was 33.1 mm, and 19.06% less than that of the control. (NH4)2SO4 obviously inhibited the growth of C913 hyphae. According to the morphological characteristics of the strain and the results of 18S rDNA-ITS sequence analysis, the strain C913 was identified as Colletotrichum gloeosporioides of the genus Colletotrichum of the subphylum Coelospora. The pathogen is found on diseased leaves of watermelon for the first time. When the temperature is 20-31℃ and pH is 6, it is the most suitable condition for mycelial growth, and when the temperature is 25-31℃ and pH is 9, it is the most suitable condition for sporulation. The colony diameter is the largest in the medium with glucose and sucrose as carbon sources, and the colony diameter is in the order of peptone>NaNO3>glycine>glutamic acid>agar>(NH4)2SO4. |
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| Keywords: | watermelon anthracnose 18S rDNA-ITS biological characteristics Colletotrichum gloeosporioides colony diameter |
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