| 18个茶树菇菌株的亲缘关系分析及农艺性状评价 |
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| 引用本文： | 王洪秀,孙鹏,安颖,胡佳,陈绪涛,李菁,魏云辉.18个茶树菇菌株的亲缘关系分析及农艺性状评价[J].中国农学通报,2022,38(1):23-31. |
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| 作者姓名： | 王洪秀 孙鹏 安颖 胡佳 陈绪涛 李菁 魏云辉 |
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| 作者单位： | 1.江西省农业科学院农业应用微生物研究所,南昌 330200;2.浙江大学生命科学学院生物物理研究所/教育部生物系统稳态与保护重点实验室,杭州 310058 |
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| 基金项目： | 国家现代农业产业技术体系建设专项“南昌综合试验站”(CARS-20)；;国家自然科学基金项目“橘小实蝇肠道引诱菌的筛选及其对宿主影响的研究”(31460490)； |
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| 摘 要： | 为获得不同来源茶树菇的亲缘关系并筛选适合工厂化生产综合农艺性状较优菌株,以18株(4株野生菌株、7株栽培菌株、5株经钴60辐照诱变菌株和2株搭载神州十号的航天飞船诱变菌株)茶树菇菌株为试验材料,采用分子标记ISSR、拮抗和同工酶技术进行菌株分型和亲缘关系分析;通过分析菌盖厚度和直径、菌柄长度和直径、生育期、产量,对18...
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| 关 键 词： | 茶树菇 拮抗反应 简单序列重复区间 酯酶同工酶 亲缘关系 农艺性状 |
| 收稿时间： | 2021-04-25 |
Genetic Relationship Analysis and Agronomic Traits Evaluation of 18 Agrocybe aegerita Strains |
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| WANG Hongxiu,SUN Peng,AN Ying,HU Jia,CHEN Xutao,LI Jing,WEI Yunhui.Genetic Relationship Analysis and Agronomic Traits Evaluation of 18 Agrocybe aegerita Strains[J].Chinese Agricultural Science Bulletin,2022,38(1):23-31. |
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| Authors: | WANG Hongxiu SUN Peng AN Ying HU Jia CHEN Xutao LI Jing WEI Yunhui |
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| Institution: | 1.Institute of Agricultural Applied Microbiology, Jiangxi Academy of Agricultural Sciences, Nanchang 330200;2.MOE Key Laboratory of Biosystems Homeostasis & Protection/ Institute of Biophysics, College of Life Sciences, Zhejiang University, Hangzhou 310058 |
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| Abstract: | In this study, 18 strains of Agrocybe aegerita (4 wild strains, 7 commercially cultivated strains, 5 strains mutated by cobalt-60 irradiation and 2 mutagenesis strains from Shenzhou 10 spacecraft) were used as experimental materials, to study the genetic relationship of Agrocybe aegerita and screen out strains with better agronomic traits for industrial production. Molecular markers ISSR, antagonistic test and isozyme techniques were used to analyze the genotyping and genetic relationship of the strains. By analyzing the cap thickness and diameter, stalk length and diameter, growth period and yield, the agronomic traits of the 18 strains of Agrocybe aegerita were compared. The 18 strains of Agrocybe aegerita were divided into 7 groups by antagonistic test. The 20 ISSR primers screened out could amplify 164 clear bands, and the average ratio of polymorphic bands was 81.1%. Clustering analysis of ISSR markers showed that the 18 strains could be divided into 6 groups when the genetic similarity coefficient was 0.828. The 18 strains were also divided into 6 groups by esterase isozyme test. The agronomic traits analysis showed that ‘Cha 5’, ‘AS-1’, ‘Cha 3’, ‘Baicha’, ‘Chayuan 1’ and ‘Yangshudong 1’ had better comprehensive traits. The results of ISSR molecular marker test, antagonistic test and esterase isozyme test were basically consistent. The optimal strains ‘Chayuan 1’, ‘Yangshudong 1’, ‘AS-1’, ‘Cha 3’, ‘Baicha’ and ‘Cha 5’ were selected as the industrial production strains of Agrocybe aegerita. |
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| Keywords: | Agrocybe aegerita antagonistic reaction ISSR esterase isoenzyme genetic relationship agronomic traits |
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