| 绿色荧光蛋白标记解淀粉芽孢杆菌Bam22在油菜体内的定殖 |
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| 引用本文： | 杨潇湘,黄小琴,张蕾,张重梅,鲜贇曦,周西全,刘勇.绿色荧光蛋白标记解淀粉芽孢杆菌Bam22在油菜体内的定殖[J].中国农学通报,2022,38(1):125-130. |
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| 作者姓名： | 杨潇湘 黄小琴 张蕾 张重梅 鲜贇曦 周西全 刘勇 |
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| 作者单位： | 1.四川省农业科学院植物保护研究所,成都 610066;2.农业农村部西南作物有害生物综合治理重点实验室,成都 610066 |
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| 基金项目： | 国家现代农业产业技术体系四川油菜创新团队(sccxtd-2021-03)； |
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| 摘 要： | 研究GFP标记的解淀粉芽孢杆菌Bam22在油菜植株上的定殖规律,为其开发利用提供科学依据.采用自然转化法将携带绿色荧光蛋白基因的质粒pGFP4412导入Bam22细胞中,构建GFP荧光标记菌株;对比野生型菌株Bam22与标记菌株Bam22-GFP的生长曲线和抑菌能力;通过灌根法,观察标记菌株在油菜体内不同组织部位的定殖...
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| 关 键 词： | 油菜 解淀粉芽孢杆菌 绿色荧光蛋白标记 定殖 根肿菌 |
| 收稿时间： | 2021-08-31 |
Colonization of GFP-labelled Bacillus amyloliquefaciens Bam22 in Brassica napus |
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| YANG Xiaoxiang,HUANG Xiaoqin,ZHANG Lei,ZHANG Zhongmei,XIAN Yunxi,ZHOU Xiquan,LIU Yong.Colonization of GFP-labelled Bacillus amyloliquefaciens Bam22 in Brassica napus[J].Chinese Agricultural Science Bulletin,2022,38(1):125-130. |
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| Authors: | YANG Xiaoxiang HUANG Xiaoqin ZHANG Lei ZHANG Zhongmei XIAN Yunxi ZHOU Xiquan LIU Yong |
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| Institution: | 1.Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066;2.Key Laboratory of Integrated Pest Management in Southwest Agriculture Crops, Ministry of Agriculture and Rural Affairs, Chengdu 610066 |
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| Abstract: | The paper aims to investigate the colonization of GFP-labelled Bacillus amyloliquefaciens Bam22 in Brassica napus and lay a solid foundation for the application of this biocontrol agent. In this study, plasmid pGFP4412 containing green fluorescent protein gene was introduced into Bam22 cells by natural transformation method to construct GFP fluorescent labelled strain. The growth curve and inhibition activity to pathogenic microorganism of wild type strain Bam22 and Bam22-GFP were compared. The number of colonized strains in different tissue parts of B. napus was observed, and the colonization rule and ability were analyzed. The results showed that the labelled strain Bam22-GFP emitted strong green fluorescence under the excitation light wavelength of 488 nm, and the GFP fluorescent protein labelling and its gene expression did not affect the growth curve and inhibition activity to pathogenic microorganism of Bam22. The labelled strain could colonize in the B. napus plant through the root irrigation, and was transmitted from the root to the stem and leaf of B. napus. The colonization rules of Bam22-GFP in the root, stem and leaf all showed a trend of first increase and then decrease. On the 45 th day after inoculation, the labelled strains could still be detected in the roots, stems and leaves of B. napus. The results show that Bam22 could colonize and transfer in B. napus by root irrigation and display good colonization ability, and it has a good application prospect in agricultural production. |
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| Keywords: | Brassica napus Bacillus amyloliquefaciens green fluorescence protein labelling colonization Plasmodiophora brassicae |
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