| 快速检测痕量汞的光电型传感方法的建立 |
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| 引用本文： | 商璟,翁芝莹,柴春彦,张洪才,刘国艳.快速检测痕量汞的光电型传感方法的建立[J].上海交通大学学报(农业科学版),2012,30(2):56-59. |
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| 作者姓名： | 商璟 翁芝莹 柴春彦 张洪才 刘国艳 |
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| 作者单位： | 1. 上海交通大学农业与生物学院
2. 上海交通大学农业与生物学院;上海市兽医生物技术重点实验室,上海200240 |
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| 基金项目： | 上海市科委技术标准专项(06DZ050360) |
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| 摘 要： | 为建立一种检测痕量汞的光电型传感方法,利用汞抑制葡萄糖氧化酶体系(即葡萄糖氧化酶—辣根过氧化物酶-葡萄糖-邻联甲苯胺—偶联反应体系)生成蓝色产物的原理,将Glu与OT固定化制成试纸条,插入自制的便携式光电型传感器,绘制出汞离子溶液浓度与对应的反射光强度值的标准曲线。实验结果表明,当汞溶液的浓度范围分别处于0.010～0.050mmoL/L时,光反射强度值与汞浓度皆呈现良好线性关系。经优化检测条件,汞溶液与双酶孵育的最佳时间为1h。该方法测定汞的检出限为1.0×10-4 mmol/L,检测时间分别为5min。由此可知,此方法检测汞快速、简便,有望实现对样品中汞含量的现场检测。
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| 关 键 词： | 光电传感器 汞 葡萄糖氧化酶—辣根过氧化物酶-葡萄糖—邻联甲苯胺 |
Development of Photoelectrical Sensor Method for Determination of Mercury |
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| SHANG Jing
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WENG Zhi-ying
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CHAI Chun yan
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ZHANG Hong-cai
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LIU Guo-yan.Development of Photoelectrical Sensor Method for Determination of Mercury[J].Journal of Shanghai Jiaotong University (Agricultural Science),2012,30(2):56-59. |
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| Authors: | SHANG Jing WENG Zhi-ying CHAI Chun yan ZHANG Hong-cai LIU Guo-yan |
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| Institution: | 1,2 (1.School of Agriculture and Biology,Shanghai Jiaotong University; 2.Key Laboratory of Shanghai Veterinary Biotechnology,Shanghai 200240,China) |
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| Abstract: | Based on the principle that mercury inhibits glucose oxidase and Glucose oxidase-horseradish peroxidase-glucose-o-tolidine coupling reaction system and produces blue product,a kind of test strip for detecting trace mercury was developed by immobilizing glucose and o-tolidine.Once the test strip was inserted into a self-made photoelectrical sensor,the light reflection intensity was recorded and shown by the sensor.A standard calibration curve was established between light reflection intensity and mercury levels,which showed a good linear relationship when the range of mercury concentration was 0.010~0.050 mmol/L.By this method,the high point for incubation of mercury solution and double enzymes was 1 hour.The detection limit can be achieved 1.0×10-4 mmol/L,and the detection time was within 5 min.The method is quick and simple to be performed.Hopefully,the sensor can be used on-site inspection of mercury pollution. |
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| Keywords: | photoelectrical biosensor mercury glucose oxidase-horseradish peroxidase-glucose-o-tolidine |
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