| 荞麦总黄酮提取工艺研究 |
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| 引用本文： | 李盈蕾,陈建华.荞麦总黄酮提取工艺研究[J].安徽农业科学,2008,36(6):2201-2202. |
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| 作者姓名： | 李盈蕾 陈建华 |
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| 作者单位： | 1. 淮海工学院化学工程系,江苏连云港,222005
2. 淮海工学院江苏省海洋生物技术重点建设实验室,江苏连云港,222005 |
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| 基金项目： | 江苏省海洋生物技术重点实验室资助项目(2006HS018) |
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| 摘 要： | 目的]优选提取荞麦粉总黄酮的工艺条件。方法]在标准液的制备及标准曲线的制作基础上,选用4个试验因素(浸提温度、乙醇浓度、浸提时间及乙醇用量)进行3水平正交试验优选,确定提取荞麦总黄酮的最佳工艺参数。结果]4种因素对荞麦粉中总黄酮提取结果影响依次为:提取时间>提取温度>乙醇浓度>乙醇用量;总黄酮提取的最佳工艺条件为:10倍体积的75%乙醇溶液在65℃下浸提4 h。在此工艺条件下,荞麦总黄酮得率平均为2.91%。荞麦总黄酮的加样回收率为97.41%,RSD为0.847 2%,其吸光度在1 h内稳定,操作过程不影响测定结果。结论]该试验优选的提取工艺结果重复性好,回收率高,简单可行。
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| 关 键 词： | 荞麦总黄酮 提取 正交试验 工艺研究 |
| 文章编号： | 0517-6611(2008)06-02201-02 |
| 修稿时间： | 2007年10月30 |
Study on the Extraction Technology of Total Flavones from Buckwheat |
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| LI Ying-lei.Study on the Extraction Technology of Total Flavones from Buckwheat[J].Journal of Anhui Agricultural Sciences,2008,36(6):2201-2202. |
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| Authors: | LI Ying-lei |
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| Abstract: | Objective] The study aimed to optimize the technological condition of extracting total flavones from buckwheat powder.Method] Based on preparing standard solution and making standard curve,four experimental factors(extraction temperature and time,ethanol concentration and dosage) were selected to conduct the orthogonal optimization experiment with 3 levels so as to ascertain the optimum technological parameters of extracting total flavones from buckwheat.Result] The influences of 4 factors on the extraction results of total flavones from buckwheat powder in order were extraction time > extraction temperature > ethanol concentration > ethanol dosage.The optimum technological condition of extracting total flavones was extraction with 10 times volume of 75% ethanol solution at 65 ℃ for 4 h,under which the average yield of total flavones from buckwheat was 2.91%.The average recovery of total flavones from buckwheat was 97.41% with RSD of 0.847 2%,its absorbency would be stable in 1 h and the operation course didn't influence detection results.Conclusion] The extraction technology optimized from this experiment had good repeatability on results,higher recovery and was simple and feasible. |
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| Keywords: | Buckwheat-total flavones Extraction Orthogonal experiment Technological study |
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