Identification of Resistance Genes Against Powdery Mildew in Four Accessions of Hordeum Vulgare SSP. Spontaneum |
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| Authors: | Jana ?epková Antonín Dreiseitl Pavel Lízal Zdeňka Kyjovská Kate?ina Teturová Radka Psotková Ahmed Jahoor |
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| Institution: | (1) Faculty of Sciences, Department of Genetics and Molecular Biology, Masaryk University Brno, Kotlářská 2, CZ-61137 Brno, Czech Republic;(2) Agricultural Research Institute Kroměříž Ltd., Havlíčkova 2787, CZ-76701 Kroměříž, Czech Republic;(3) Department of Agricultural Sciences, Plant and Soil Science Laboratory, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, 1871 Frederiksberg, Copenhagen, Denmark |
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| Abstract: | Summary Four newly detected accessions of wild barley (Hordeum
vulgare ssp. spontaneum) resistant to powdery mildew caused by Blumeria
graminis f. sp. hordei were studied with the aim of finding the number of genes/loci conferring the resistance of individual accessions, the type of inheritance of the genes and their relationships to the Mla locus. F2 populations after crosses between the winter variety ‘Tiffany’ and four wild barley accessions and use of microsatellite DNA markers were focused on the identification of individual resistance genes/loci by means of their chromosomal locations. In PI466495, one locus conferring powdery mildew resistance was identified in highly significant linkage with the marker Bmac0213. This location is consistent with the known locus Mla on chromosome 1HS. In the other three accessions the resistance was determined by two independent loci. In PI466197, PI466297 and PI466461, one locus was identified on chromosome 1HS and three new loci were revealed on chromosomes 2HS (highly significant linkage with Bmac0134), 7HS (highly significant linkage with Bmag0021) and 7HL (significant linkage with EBmac0755). Our prospective aim is identification of further linked DNA markers and the exact location of the resistance genes on the barley chromosomes. |
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| Keywords: | barley Blumeria graminis f sp hordei Hordeum vulgare ssp spontaneum microsatellites powdery mildew resistance genes |
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