| 番鸭细小病毒强毒株(MDPV-Q)VP_2基因的序列分析 |
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| 引用本文： | 娄华,白挨泉,顾万军,陆英杰,杨德威,刘福安.番鸭细小病毒强毒株(MDPV-Q)VP_2基因的序列分析[J].中国预防兽医学报,2001(5). |
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| 作者姓名： | 娄华 白挨泉 顾万军 陆英杰 杨德威 刘福安 |
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| 作者单位： | 佛山科技学院动物医学系 广东南海528231
(娄华，白挨泉，顾万军，陆英杰)，华南农业大学动物医学系 广东广州510642
(杨德威)，华南农业大学动物医学系 广东广州510642(刘福安) |
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| 摘 要： | 应用PCR技术扩增了MDPV_Q株的VP2 蛋白基因片段。将扩增后的VP2 基因重组到pMD18_T质粒载体上 ,并对插入片段进行序列测定。将测序结果及由该结果推导的氨基酸序列分别与MDPV、GPV进行了同源性比较及分析。结果表明 ,我国分离的MDPV_Q株其VP2 基因序列与国外分离株具有很高的同源性。而与GPV的同源性较低。
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| 关 键 词： | 番鸭细小病毒 鹅细小病毒 序列分析 |
Sequence Analysis of DNA Encoding VP_2 Structural Protein of Muscovy Duck Parvovirus Q Strain |
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| LOU Hua ,BAI Ai_quan ,GU Wan_jun,LU Ying_jie,YANG De_wei ,LIU Fu_an.Sequence Analysis of DNA Encoding VP_2 Structural Protein of Muscovy Duck Parvovirus Q Strain[J].Chinese Journal of Preventive Veterinary Medicine,2001(5). |
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| Authors: | LOU Hua BAI Ai_quan GU Wan_jun LU Ying_jie YANG De_wei LIU Fu_an |
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| Institution: | LOU Hua 1,BAI Ai_quan 1,GU Wan_jun,LU Ying_jie,YANG De_wei 2,LIU Fu_an 2 |
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| Abstract: | The DNA encoding VP 2 structural protein was amplified by PCR and recombined into T vector and sequenced Compare gene and amino acid squence of VP 2 of MDVP_Q with that of GPV and MDPV which were registered in the genbank.It shows that both DNAs encoding VP 2 structural protein of MDPV_Q and reported MDPV have high homology,while the homology with GPV is low. |
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| Keywords: | Muscovy Duck parvovirus Goose parvovirus Sequence analysis |
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