| 利用多种分子标记构建龙眼高密度分子遗传图谱 |
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| 引用本文： | 郭印山,赵玉辉,刘朝吉,任鹏荣,黄天林,傅嘉欣,卢博彬,刘成明.利用多种分子标记构建龙眼高密度分子遗传图谱[J].园艺学报,2009,36(5):655-662. |
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| 作者姓名： | 郭印山 赵玉辉 刘朝吉 任鹏荣 黄天林 傅嘉欣 卢博彬 刘成明 |
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| 作者单位： | (华南农业大学园艺学院, 广州510642; 潮州市果树研究所, 广东潮州515726) |
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| 基金项目： | 国家自然科学基金，中国博士后科学基金，广东省科技计划项目 |
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| 摘 要： | 以龙眼特优质品种‘凤梨朵’为母本, 大果型主栽品种‘大乌圆’为父本, 杂交创建了‘凤梨朵’ ב大乌圆’F1群体。从该杂种群体中随机选用94个单株作为作图群体, 连同两个亲本品种, 进行了RAPD、ISSR、SRAP和AFLP分子标记分析, 并运用JoinMap3.0进行连锁分析, 分别构建了‘凤梨朵’和‘大乌圆’的分子遗传图谱, 其中, ‘凤梨朵’的遗传图谱为21个连锁群, 包含183个标记位点,覆盖总图距965.1 cM, 位点间平均遗传距离为5.84 cM; ‘大乌圆’的遗传图谱为22个连锁群, 包含251个标记位点, 覆盖总图距1 064.8 cM, 位点间平均遗传距离为4.65 cM。这是龙眼上首次报道的高密度分子遗传图谱, 为后续的基因定位及辅助选择奠定了良好的基础。
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| 关 键 词： | 龙眼 分子标记 遗传图谱 |
| 收稿时间： | 2008-10-27 |
| 修稿时间： | 2009-2-19 |
Construction of a Molecular Genetic Map for Longan Based on RAPD,ISSR.SRAP and AFLP Markers |
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| GUO Yin-shan,ZHAO Yu-hui,LIU Chao-ji,REN Peng-rong,HUANG Tian-lin,FU Jia-xin,LU Bo-bin,LIU Cheng-ming.Construction of a Molecular Genetic Map for Longan Based on RAPD,ISSR.SRAP and AFLP Markers[J].Acta Horticulturae Sinica,2009,36(5):655-662. |
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| Authors: | GUO Yin-shan ZHAO Yu-hui LIU Chao-ji REN Peng-rong HUANG Tian-lin FU Jia-xin LU Bo-bin LIU Cheng-ming |
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| Institution: | (College of Horticulture, South China Agricultural University, Guangzhou 510642, China; Chaozhou Fruit Research Institute,Chaozhou, Guangdong 515726, China) |
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| Abstract: | A big F1 population of‘Fengliduo ×Dawuyuan’were created by crossing between a high quality cultivar‘Fengliduo’and a large fruit size main cultivar‘Dawuyuan’. Among the hybrid population,94 F1 individuals were randomly chosen and used as the mapp ing population. Based on the mapp ing population, a molecular genetic linkage map of longan was constructed with RAPD, ISSR, SRAP and AFLP molecular markers. According to the linkage analysis by JoinMap3.0, the molecular linkage map of‘Fengliduo’fell into 21 linkage group s, which contained 183 markers, with an average interval of 5184 cM and covered a total distance of 965.1 cM; While the molecular linkage map of‘Dawuyuan’fell into 22 linkage group s, which contained 251 markers, with an average interval of 4.65 cM and covered a total distance of 1 064.8 cM. This was the first report about high-density molecular genetic map for longan and would lay a solid foundation for gene location and marker assited selection in longan breeding. |
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| Keywords: | longan molecular marker genetic map |
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