| 东北刺人参体细胞胚状体的诱导及体细胞胚胎发生的扫描电镜观察 |
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| 引用本文： | 夏广清,朱俊义,王艳萍.东北刺人参体细胞胚状体的诱导及体细胞胚胎发生的扫描电镜观察[J].吉林农业大学学报,2009,31(2). |
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| 作者姓名： | 夏广清 朱俊义 王艳萍 |
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| 作者单位： | 通化师范学院生物系,通化,134001 |
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| 基金项目： | 吉林省教育厅科研项目 |
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| 摘 要： | 分别以东北刺人参叶芽和带有叶柄的幼叶为外植体,研究6-BA浓度对东北刺人参体细胞胚状体诱导的影响,并利用扫描电镜对体细胞胚胎发生的形态学进行了观察.结果表明:以带有叶柄的东北刺人参幼叶为外植体,在MS培养基上附加4.0 mg/L 6-BA和0.5 mg/L NAA,可获得健状的胚状体,并且胚状体经过球形胚、心形胚、鱼雷形胚和子叶形胚的胚胎发育时期,最终形成可发育成完整小植株的成熟胚.
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| 关 键 词： | 东北剌人参 体细胞胚状体 诱导 扫描电镜 |
Induction and Observation of the Somatic Embryogenesis of Oplopanax elatus by Scanning Electron Microscope |
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| XIA Guang-qing,ZHU Jun-yi,WANG Yan-ping.Induction and Observation of the Somatic Embryogenesis of Oplopanax elatus by Scanning Electron Microscope[J].Journal of Jilin Agricultural University,2009,31(2). |
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| Authors: | XIA Guang-qing ZHU Jun-yi WANG Yan-ping |
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| Institution: | XIA Guang-qing,ZHU Jun-yi,WANG Yan-pingTonghua Normal University,Tonghua 134001,China |
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| Abstract: | The leaves with leafstalk and buds of Oplopanax elatus Nakai were taken as explants and cultured in vitro.Large and healthy embryoids were obtained on MS medium supplement with 4.0 mg/L,0.5 mg/L NAA from buds.The development of somatic embryos of Oplopanax elatus Nakai was observed by scanning electron microscopy.The results showed that the embryoids were developed from global,heart-shaped,torpedo-shaped and cotyledon embryo,then matured and formed planet. |
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| Keywords: | Oplopanax elatus Nakai somatic embryogenesis induction scanning electron microscope |
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