Development of a reference diet for use in indispensable amino acid requirement studies of gilthead seabream Sparus aurata L. |
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| Authors: | PA Marcouli MN Alexis A Andriopoulou & J Iliopoulou-Georgudaki |
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| Institution: | Department of Biology, Section of Animal Biology, University of Patras, Patras, Greece;;National Center for Marine Research, Aghios Kosmas, Hellinikon, Athens, Greece |
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| Abstract: | The development of a test diet supporting good fish growth to be used in gilthead seabream indispensable amino acid (IAA) requirement studies based on dose–response relationship was the objective of the present study. Four isonitrogenous diets, one serving as control (diet C) based on fish meal LT, and three others in which the fish meal component was reduced to 45% and the rest of the nitrogen was provided by mixtures of crystalline AA were formulated. Diet S resembled the whole body IAA profile of wild seabream. Diet D was made deficient in all IAA and a 10% excess of IAA compared with diet S was used in diet E. Triplicate groups of fish were fed the diets to satiation for 6 weeks. Growth parameters and nutrient utilization efficiencies among diets C and S were similar, while excessive supplementation of IAA negatively affected most of them, compared with the control. Whole body composition was differentiated in relation to the diet fed. No significant differences were observed among groups fed the control and diet S. As shown by the results, the presence of crystalline AA in diet S does not significantly affect growth, and could therefore serve as control diet in IAA requirement studies of gilthead seabream. |
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| Keywords: | indispensable amino acid nutrition seabream Sparus aurata |
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