Morphological and cytogenetic studies on the hybrid between bread wheat and <Emphasis Type="Italic">Psathyrostachys huashanica</Emphasis> Keng ex Kuo |
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| Authors: | Hou-Yang Kang Hai-Qin Zhang Xing Fan Yong-Hong Zhou |
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| Institution: | (1) Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan, 611830, Sichuan, China;(2) Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Yaan, 625014, Sichuan, China |
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| Abstract: | Psathyrostachys huashanica Keng ex Kuo (2n = 2x = 14, NsNs), a source of wheat stripe rust, take-all fungus, and powdery mildew resistance with tolerance to salinity and drought, has been successfully hybridized as the
pollen parent to bread wheat without using immature embryo rescuing culture for the first time. All of the CSph2b × P. huashanica hybrid seeds germinate well. Backcross derivatives were successfully obtained. F1 hybrids were verified as intergeneric hybrids on the basis of morphological observation, cytological and molecular analyses.
The results obviously showed the phenotypes of the hybrid plants were intermediate between bread wheat and P. huashanica. Chromosome pairing at MI of PMCs in the F1 hybrid plants was low, and the meiotic configuration was 26.80 I + 0.60 II (rod). Cytological analysis of the hybrid plants
revealed the ineffectiveness of the ph2b gene on chromosome association between the parents. Eight RAPD-specific markers for Ns genome were selected for RAPD analysis, and the results indicated that F1 hybrids contained the Ns genome of P. huashanica. Furthermore, the significance of the finding for bread wheat improvement was discussed. |
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| Keywords: | Bread wheat Cytology Genetic improvement Morphology Psathyrostachys huashanica RAPD |
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