| 宏基因组学及其在瘤胃微生物中的应用研究进展 |
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| 引用本文： | 吴锡川,杨舒黎,苟潇,冷静,毛华明.宏基因组学及其在瘤胃微生物中的应用研究进展[J].中国畜牧兽医,2011,38(12):106-110. |
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| 作者姓名： | 吴锡川 杨舒黎 苟潇 冷静 毛华明 |
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| 作者单位： | 云南农业大学云南省动物营养与饲料重点实验室,云南昆明 650201 |
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| 基金项目： | 国家自然科学基金，云南省自然科学基金 |
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| 摘 要： | 宏基因组学是研究生态群体基因功能的一门崭新的学科。它通过免培方法获得微生物的纯培养,主要技术包括DNA的提取、文库的构建和目标基因克隆的筛选,可用于发现新基因、开发新的生物活性物质、研究群落中微生物多样性等方面。文章介绍了宏基因组学的基本方法,并对瘤胃微生物宏基因组学的应用现状进行了综述。
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| 关 键 词： | 宏基因组学 瘤胃微生态 文库构建 |
Metagenomics and Its New Progress in the Research of Rumen Microbiota |
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| WU Xi-chuan
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YANG Shu-li
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GOU Xiao
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LENG Jing
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MAO Hua-ming.Metagenomics and Its New Progress in the Research of Rumen Microbiota[J].China Animal Husbandry & Veterinary Medicine,2011,38(12):106-110. |
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| Authors: | WU Xi-chuan YANG Shu-li GOU Xiao LENG Jing MAO Hua-ming |
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| Institution: | Animal Nutrtion and Feed Laboratory of Yunnan Province, Yunnan Agricultural University, Kunming 650201,China |
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| Abstract: | Metagenomics based on study of ecological community gene function is a brand-new discipline. It tries to use culture-independent approaches,and it offers enormous scope and potential for microbiology. It comprises isolation of DNA,library construction and screening of target gene clone,and can be used in the discovery of new gene and biocatalysts and in the study of microbial biodiversity in a community.The methodology of metagenomics and the application of metagenomics in rumen have been briefly summarized. |
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| Keywords: | metagenomics rumen microbiota library constructing |
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