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| 致犊牛肺炎和关节炎牛支原体新疆株的分离与鉴定 | |
| 引用本文： | 姚永进,剡根强,王静梅,范伟兴,李岩.致犊牛肺炎和关节炎牛支原体新疆株的分离与鉴定[J].中国畜牧兽医,2011,38(12):76-78. |
| 作者姓名： | 姚永进 剡根强 王静梅 范伟兴 李岩 |
| 作者单位： | 1. 石河子大学动物科技学院,新疆石河子,832003 2. 中国动物卫生与流行病学中心,山东青岛,266032 3. 新疆生产建设兵团畜牧兽医工作总站,新疆乌鲁木齐,830052 |
| 摘 要： | 自2010年7月以来,新疆北疆部分地区某些奶牛场犊牛陆续出现严重的肺炎和关节炎,为确定其病原,无菌采集8个牛场病死犊牛的肺脏和关节液,患病犊牛的血清和部分鼻拭子,病料接种筛选培养基,通过菌落形态观察、特异性PCR鉴定、生长抑制试验和血清学检测,确定牛支原体15株,其中7株牛支原体克隆测序,与PG45 OPP F基因同源性为97.32%~100.00%。牛支原体ELISA试剂盒检测92份血清显示,阳性率82.61%。结果表明,该地区奶牛场发病犊牛以牛支原体感染为主。 |
| 关 键 词： | 犊牛 肺炎 关节炎 牛支原体 分离 鉴定 |
| 收稿时间： | 2011-05-05 |
Isolation and Identification of Mycoplasma bovis Xinjiang Strain Inducing Pneumonia and Arthritis of Calf |
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| YAO Yong-jin , YAN Gen-qiang , WANG Jing-mei , FAN Wei-xing , LI Yan.Isolation and Identification of Mycoplasma bovis Xinjiang Strain Inducing Pneumonia and Arthritis of Calf[J].China Animal Husbandry & Veterinary Medicine,2011,38(12):76-78. | |
| Authors: | YAO Yong-jin YAN Gen-qiang WANG Jing-mei FAN Wei-xing LI Yan |
| Institution: | 1. College of Animal Science and Technology, Shihezi University, Shihezi 832003,China;2. China Animal Health and Epidemiology Center, Qingdao 266032, China;3. Production and Construction Corps Animal Husbandryand Veterinary Primal Station, Urumqi 830052, China |
| Abstract: | Since July 2010, a serious pneumonia and arthritis occurred in the calves of some dairies in north area of Xinjiang. In order to detect the pathogen, the died calf lungs and joint fluid, the sick calf serum and some nasal swabs was collected with asepsis from 8 dairies. Add the samples was inoculated the selected material medium. The 15 Mycoplasma bovis(M.bovis) isolates were identified by colony morphology, specific PCR, growth inhibition test (GIT) and serological test, 7 isolates were cloned and sequenced, the homology of nucleotide sequences were 97.32% to 100.00% in OPP F gene with PG45 strain. Furthermore, 92 serum samples were detected by ELISA, and the positive rate was 82.61%. The results indicated that the disease was primarily caused by M.bovis. |
| Keywords: | calf pneumonia arthritis Mycoplasma bovis isolation identification |
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