| 豆类丝核菌次级代谢产物与阿霉素的体外联合抑瘤作用研究 |
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| 引用本文： | 杨萍,武学峰,姚旭辉,刘少兴,杨鸣琦.豆类丝核菌次级代谢产物与阿霉素的体外联合抑瘤作用研究[J].安徽农业科学,2010,38(3):1124-1125,1176. |
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| 作者姓名： | 杨萍 武学峰 姚旭辉 刘少兴 杨鸣琦 |
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| 作者单位： | 咸阳市动物疫病预防控制中心,陕西咸阳,712000;西北农林科技大学动物医学院,陕西杨凌,722100;咸阳市动物疫病预防控制中心,陕西咸阳,712000;咸阳市动物卫生监督所,陕西咸阳,712000;西北农林科技大学动物医学院,陕西杨凌,722100 |
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| 基金项目： | 国家自然科学基金资助项目(30270979) |
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| 摘 要： | ［目的］研究豆类丝核菌次级代谢产物与阿霉素的降毒增效作用。方法］分别采用6种不同浓度的豆类丝核菌次级代谢产物（苦马豆素含量分别为10.00、5.00、2.50、1.25、0.62、0.31 μg/ml）处理人肝癌细胞HepG2，测量其IC50；然后用此代谢产物与2种不同浓度（60、6 μg/ml）阿霉素（ADR）联合处理HepG2，分析两者的增效作用。结果］豆类丝核菌次级代谢产物可抑制肝癌细胞HepG2生长，其对HepG2细胞作用72 h的IC50为1.25 μg/ml；ADR可显著抑制肝癌细胞HepG2生长，加入豆类丝核菌次级代谢产物后HepG2细胞的形态和存活率变化不大。结论］豆类丝核菌次级代谢产物与阿霉素体外联合不具有增效作用。
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| 关 键 词： | 豆类丝核菌 阿霉素 HepG2 |
Study on Anti-tumor Effect of Secondary Metabolites of Rhizotonia legumeinicola Combined with Adriamycin on HepG2 in Vitro |
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| YANG Ping et al.Study on Anti-tumor Effect of Secondary Metabolites of Rhizotonia legumeinicola Combined with Adriamycin on HepG2 in Vitro[J].Journal of Anhui Agricultural Sciences,2010,38(3):1124-1125,1176. |
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| Authors: | YANG Ping |
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| Institution: | Xianyang Animal Epidemic Disease Prevention and Control Center;Xianyang;Shaanxi 712000 |
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| Abstract: | Objective] The aim was to study the synergism of secondary metabolites of Rhizotonia legumeinicola and adriamycin. Method] The secondary metabolites of R. legumeinicola with different concn. (10.00, 5.00, 2.50, 1.25, 0.62, 0.31μg/ml) was taken to treat HepG2 and its IC_(50) was determined. And then the HepG2 cells were treated by the secondary metabolites combined with ADR with different concn. (60, 6 μg/ml), and their synergism were studied. Result] The secondary metabolites of R. legumeinicola could inhibit the growth of HepG2 and its IC_(50) on HepG2 after 72 h was 1.25 μg/ml. The ADR could significantly inhibit the growth of HepG2, and the morphology and survival rate of HepG2 cells changed little after addition of the secondary metabolites of R. legumeinicola. Conclusion] The secondary metabolites of R. legumeinicola combined with adriamycin had no synergism in vitro. |
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| Keywords: | Rhizotonia legumeinicola Adriamycin HepG2 |
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