Survival,growth and fertility of gynogenetic diploids induced in the cyprinid loach, Misgurnus anguillicaudatus |
| |
| Authors: | Ryo Suzuki Takashi Oshiro Teruyuki Nakanishi |
| |
| Institution: | National Research Institute of Aquaculture, Nansei, Mie, 516-01 Japan |
| |
| Abstract: | Gynogenetic diploids were induced in the loach, Misgurnus anguillicaudatus, by applying cold shock to the egg after fertilization with spermatozoa genetically inactivated by ultraviolet-ray irradiation, to examine their survival, growth and fertility. Eggs were obtained from orange phenotype loach (homozygous for a recessive demelanogenesis gene). Spermatozoa were obtained from black phenotype loach (homozygous for a dominant melanogenesis gene), and from common carp, Cyprinus carpio. All hybrids between loach and carp were inviably abnormal.Eggs fertilized with loach or carp spermatozoa irradiated at doses of 6000 and 12000 erg/mm 2 and then treated with cold water (1°C for one hour 4 and 5 min after fertilization developed into embryos with 61–78% survival. About 70–87% of newly-hatched fry were normal in appearance. Gynogenetic fish induced by both loach and carp spermatozoa showed lower rates of survival and growth than did the normal diploid controls. All gynogenetic fish from carp spermatozoa were female and 94.9% of the gynogenetic fish from loach spermatozoa were female. The second gynogenetic offsprings were produced from females selected from both gynogenetic groups. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
