| 晋豆24~(60)Coγ射线诱变后代种子贮藏蛋白的研究 |
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| 引用本文： | 李慧峰,李贵全,李玲.晋豆24~(60)Coγ射线诱变后代种子贮藏蛋白的研究[J].核农学报,2008,22(3):260-264. |
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| 作者姓名： | 李慧峰 李贵全 李玲 |
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| 作者单位： | 山西农业大学农学院,山西,太谷,030801 |
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| 基金项目： | 国家科技支撑计划"农林动植物育种工程"重大项目 |
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| 摘 要： | 选用晋豆24及其60Coγ射线辐照诱变后代为试验材料,利用SDS-PAGE聚丙烯酰胺凝胶电泳研究了诱变后代大豆贮藏蛋白亚基11S和7S的组分含量、亚基组成和各亚基的含量及其变异规律。结果表明:大豆贮藏蛋白种质资源中,同一品种的不同亚基和不同品种的同一亚基间均有较大变异;11S球蛋白与7S球蛋白呈极显著负相关,与11S/7S比值呈极显著正相关;聚类分析根据11S/7S比值将诱变后代群体分为比值较高(平均为1.70)、居中(平均为1.25)和较低(平均为0.79)3类,为诱变育种选育优质专用大豆新品种提供了依据。
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| 关 键 词： | 大豆 诱变 SDS-PAGE 贮藏蛋白亚基 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
STUDY ON SEED STORAGE PROTEIN OF OFFSPRING ~(60)Co γ-RAYS INDUCED MUTANTS OF SOYBEAN JINDOU-24 |
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| LI Hui-feng,LI Gui-quan,LI Ling.STUDY ON SEED STORAGE PROTEIN OF OFFSPRING ~(60)Co γ-RAYS INDUCED MUTANTS OF SOYBEAN JINDOU-24[J].Acta Agriculturae Nucleatae Sinica,2008,22(3):260-264. |
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| Authors: | LI Hui-feng LI Gui-quan LI Ling |
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| Abstract: | Seed of soybean variety Jindou-24 were irradiated with ~(60)Co γ-rays and the offspring of mutants were used to research the relative contents of glycinin(11S) and β-conglycinin(7S),the composition of its subunits,the contents of different subunits and the rules of its variance by using SDS-PAGE technique.The results indicated that the content of different subunits or the same subunit of different varieties varied greatly in the offspring of mutants.Significant difference existed in the contents of different subunits of the same variety or the same subunits of different varieties.The content of 11S negatively correlated with that of 7S,and positively correlated with 11S/7S.Cluster analysis showed the mutants were divided into three groups(average 11S/7S rate 1.70,1.25 and 0.79) according to the rate of 11S/7S.The results could provide theoretical guidance to breed new soybean varieties with the character of high quality and other special purpose. |
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| Keywords: | soybean induce SDS-PAGE storage protein subunit |
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