| 中国西北内陆干旱区大叶白麻野生居群遗传多样性研究(英文) |
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| 引用本文： | 赵金凤,张卫明,彭雪梅,唐泽紫,顾龚平,陆长梅.中国西北内陆干旱区大叶白麻野生居群遗传多样性研究(英文)[J].农业科学与技术,2008,9(4):39-43. |
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| 作者姓名： | 赵金凤 张卫明 彭雪梅 唐泽紫 顾龚平 陆长梅 |
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| 作者单位： | 南京师范大学生命科学学院;南京野生植物综合利用研究院 |
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| 基金项目： | 国家科技攻关项目，江苏省高新技术项目 |
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| 摘 要： | Objective] Study on the genetic diversity in wild populations of Poacynum hendersonii.Method] Random amplified polymorphic DNA(RAPD)technique was employed to analyze the genetic diversity in five wild populations of P.hendersonii sampled from Xinjiang,Gansu and Qinghai provinces.Result] Totally 165 clear and repeatable bands were generated in RAPD reaction by using 20 primers screened from 80 primers,of which 110 were polymorphic,accounting for 66.67%.At species level,Nei’s gene diversity index(H),Shannon’s information index(I)and genetic differentiation coefficient(Gst)were 0.220 5,0.304 7 and 0.908 2,respectively.P.hendersonii germplasm resources share a high level of genetic diversity,and genetic differentiation mainly exists among the populations.Results from genetic distances and cluster analysis showed that relationships among P.hendersonii populations were to some extent related with their geographical and climatic characters.Conclusion] This study suggests that the conservation of P.hendersonii should focus on the protection of many populations,particularly the Qinghai population.
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| 关 键 词： | Poacynum hendersonii Wild population Genetic diversity RAPD |
Genetic Diversity in Wild Populations of Poacynum hendersonii from Inland Arid Regions of Northwest China |
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| ZHAO Jin-feng,ZHANG Wei-ming,PENG Xue-mei,TANG Ze-zi,GU Gong-ping,LU Chang-mei.Genetic Diversity in Wild Populations of Poacynum hendersonii from Inland Arid Regions of Northwest China[J].Agricultural Science ＆ Technology,2008,9(4):39-43. |
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| Authors: | ZHAO Jin-feng ZHANG Wei-ming PENG Xue-mei TANG Ze-zi GU Gong-ping LU Chang-mei |
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| Institution: | 1 College of Life Sciences,Nanjing Normal University Nanjing 210046 Nanjing Institute for Comprehensive Utilization of Wild Plants |
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| Abstract: | Objective] Study on the genetic diversity in wild populations of Poacynum hendersonii. Method] Random amplified polymorphic DNA (RAPD) technique was employed to analyze the genetic diversity in five wild populations of P. hendersonii sampled from Xinjiang, Gansu and Qinghai provinces. Result] Totally 165 clear and repeatable bands were generated in RAPD reaction by using 20 primers screened from 80 primers, of which 110 were polymorphic, accounting for 66.67%. At species level, Nei's gene diversity index (H), Shannon's information index (I) and genetic differentiation coefficient (Gst) were 0.220 5, 0.304 7 and 0.908 2, respectively. P. hendersonii germplasm resources share a high level of genetic diversity, and genetic differentiation mainly exists among the populations. Results from genetic distances and cluster analysis showed that relationships among P. hendersonii populations were to some extent related with their geographical and climatic characters. Conclusion] This study suggests that the conservation of P. hendersonii should focus on the protection of many populations, particularly the Qinghai population. |
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| Keywords: | Poacynum hendersonii Wild population Genetic diversity RAPD |
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