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| 响应面法优化针叶樱桃总黄酮的提取工艺及其抗氧化活性研究 | |
| 引用本文： | 杨志,李文义,高云涛,熊华斌,陈毅坚,杨慧娟.响应面法优化针叶樱桃总黄酮的提取工艺及其抗氧化活性研究[J].浙江农业学报,2020,32(10):1866. |
| 作者姓名： | 杨志 李文义 高云涛 熊华斌 陈毅坚 杨慧娟 |
| 作者单位： | 1.云南民族大学 云南省跨境民族地区生物质资源清洁利用国际联合研究中心,云南 昆明 650500;2.云南民族大学 民族药资源化学国家民委-教育部重点实验室,云南 昆明 650500;3.中国计量大学 标准化学院,浙江 杭州 310018 |
| 基金项目： | 云南省应用基础研究计划(2016FD057,2017FD117); 浙江绍兴上虞标准化研究院项目 |
| 摘 要： | 为有效提取针叶樱桃中的总黄酮,在单因素实验基础上,采用响应面法研究樱桃破碎目数、乙醇体积分数、液料比对黄酮提取率的影响,并对提取的黄酮进行抗氧化活性评价。结果表明:最佳提取工艺为樱桃破碎目数63.13、乙醇体积分数49.71%、液料比29.85,此时黄酮提取率预测值为85.07 mg·100g-1,与验证实验值86.9 mg·100g-1相符,说明响应模型方法可靠;且提取率越高,提取液抗氧化活性越好,最佳提取工艺下的DPPH抗氧化当量为73.38。 |
| 关 键 词： | 针叶樱桃 总黄酮 响应面法 抗氧化活性 |
| 收稿时间： | 2020-08-04 |
Optimization of extraction process of total flavonoids from Acerola cherry by response surface methodology and their antioxidant activities |
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| YANG Zhi,LI Wenyi,GAO Yuntao,XIONG Huabin,CHEN Yijian,YANG Huijuan.Optimization of extraction process of total flavonoids from Acerola cherry by response surface methodology and their antioxidant activities[J].Acta Agriculturae Zhejiangensis,2020,32(10):1866. | |
| Authors: | YANG Zhi LI Wenyi GAO Yuntao XIONG Huabin CHEN Yijian YANG Huijuan |
| Institution: | 1. Joint Research Centre for International Cross-Border Ethnic Regions Biomass Clean Utilization in Yunnan, Yunnan Minzu University, Kunming 650500, China; 2. Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission and Ministry of Education, Yunnan Minzu University, Kunming 650500, China; 3. College of Standardization, China Jiliang University, Hangzhou 310018, China |
| Abstract: | For effective extraction of total flavonoids from Acerola cherry, on the basis of single factor experiments, the effect of cherry crushing mesh number, ethanol concentration, and liquid-solid ratio were studied by response surface methodology. Meanwhile, the antioxidant activity of total flavonoids from Acerola cherry was assessed. The results showed that the optimum extraction conditions were: cherry crushing mesh number of 63.13, ethanol volume fraction of 49.71%, liquid-solid ratio of 29.85. Under the optimal conditions, flavonoids extraction yield was 86.9 mg·100g-1 and was closed to the predicted value of 85.07 mg·100g-1. The antioxidant activity of the extracts increased with the increase of extraction rate, and the DPPH antioxidant equivalent under the optimum extraction conditions was 73.38. |
| Keywords: | Acerola cherry total flavonoids response surface methodology antioxidant activity |
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