| 锈色粒肩天牛幼虫的COⅠ、COⅡ、Cytb和28S基因片段序列分析 |
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| 引用本文： | 叶碧欢,李海波,叶卫邦,陈友吾.锈色粒肩天牛幼虫的COⅠ、COⅡ、Cytb和28S基因片段序列分析[J].浙江农林大学学报,2020,37(2):303-310. |
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| 作者姓名： | 叶碧欢 李海波 叶卫邦 陈友吾 |
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| 作者单位： | 1.浙江省林业科学研究院, 浙江 杭州 3100232.金华森林之宝农林科技开发有限公司, 浙江 金华 321000 |
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| 基金项目： | 浙江省科技计划项目2016F30012浙江省省属科研院所资助项目2019F1068-1 |
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| 摘 要： | 目的 基于分子生物学手段，探讨可快速准确鉴别锈色粒肩天牛Apriona swainsoni幼虫与其他天牛幼虫的目标基因，为该保健昆虫的食药用安全提供保障。 方法 利用线粒体COⅠ、COⅡ、Cytb基因和28S细胞核基因的通用引物分别克隆获得4种基因片段，并进行同源序列检索、多重比对、序列信息分析及进化树构建。 结果 基因克隆最终获得锈色粒肩天牛4种基因序列的片段大小分别为817、545、434和1 088 bp。特殊位点分布结果显示:28S的保守位点占比最高，其后依次为Cytb、COⅠ和COⅡ，变异率则正好相反。COⅠ、COⅡ和Cytb基因片段的碱基组成和替换信息特点均表现为A+T含量>G+C含量，颠换高于转换，28S基因片段则表现为A+T含量 结论 COⅠ、COⅡ、Cytb和28S基因均可作为鉴定该虫的分子生物学参考依据。
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| 关 键 词： | 森林保护学 锈色粒肩天牛幼虫（斗米虫） COⅠ COⅡ Cytb 28S 序列分析 物种鉴别 |
| 收稿时间： | 2019-04-10 |
Sequence analysis of COⅠ, COⅡ, Cytb and 28S gene fragments from Apriona swainsoni larvae |
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| YE Bihuan,LI Haibo,YE Weibang,CHEN Youwu.Sequence analysis of COⅠ, COⅡ, Cytb and 28S gene fragments from Apriona swainsoni larvae[J].Journal of Zhejiang A&F University,2020,37(2):303-310. |
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| Authors: | YE Bihuan LI Haibo YE Weibang CHEN Youwu |
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| Institution: | 1.Zhejiang Academy of Forestry, Hangzhou 310023, Zhejiang, China2.Jinhua Forest Treasure Technology Development Company, Jinhua 321000, Zhejiang, China |
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| Abstract: | Objective Based on molecular biological methods, this research was intended to explore the the target genes that could identify Apriona swainsoni larvae from other Cerambycidae larvae, so as to provide a guarantee for the food and drug use of A. swainsonias larvae. Method Four different gene fragments were cloned using the general primers of COⅠ, COⅡ, Cytb and 28S genes before the homologous sequence retrieval, multiple comparison, sequence analysis and molecular phylogenetic tree construction were carried out. Result (1) The fragments of the four gene sequences obtained by gene cloning were 817, 545, 434 and 1 088 bp, respectively. (2) In light of the distribution of special loci, 28S had the highest proportion of conserved sites, followed by Cytb, COⅠ and COⅡ, while the variability rate was just the opposite. The base composition and replacement of the COⅠ, COⅡ and Cytb gene fragments were all shown as A+T contents> G+C contents, with the rate of translocation higher than that of the conversion. However, when it came to the 28S gene fragment, it was A+T cotents Conclusion COⅠ, COⅡ, Cytb and 28S genes could be used as the molecular reference for the identification of A. swainsoni larvae. |
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