| 挤压对麦糟纤维结构和固态发酵过程中pH值影响的研究 |
| |
| 引用本文： | 张礼星,王晓霞,陈廷登,章银军.挤压对麦糟纤维结构和固态发酵过程中pH值影响的研究[J].林产化学与工业,2004,24(4):81-86. |
| |
| 作者姓名： | 张礼星 王晓霞 陈廷登 章银军 |
| |
| 作者单位： | 1. 浙江工业大学,生物环境学院,浙江,杭州,310014
2. 沙斯卡彻温大学,药学系,加拿大 |
| |
| 摘 要： | 低成本纤维素酶的生产是酶法转化纤维质生物量为酒精的关键。尽管已有很多有关纤维素酶的研究报告，但底物的预处理对固态发酵过程中pH值和产酶的影响很少见诸于报道。作者研究了在固态发酵过程中，里氏木霉在未处理和经挤压处理的麦糟培养基上pH值的变化。经单螺杆和双螺杆挤压的麦糟，其纤维的结晶度变化较小。单螺杆挤压撕开了纤维结构，而双螺杆破坏并摧毁了纤维细胞的细胞壁，同时麦糟的颗粒也变得很小。两种经挤压处理的麦糟均有利于菌体的生长，并提高了产酶。以单螺杆挤压处理的麦糟为培养基时，最高FPA酶活力为182．8IU／g纤维素。
|
| 关 键 词： | 固态发酵 麦糟 纤维素酶 里氏木霉 |
EFFECTS OF EXTRUSION ON STRUCTURE OF SPENT GRAIN CELLULOSE AND pH VALUE MAINTENANCE DURING SOLID-SUBSTRATE FERMENTATION |
| |
| Abstract.EFFECTS OF EXTRUSION ON STRUCTURE OF SPENT GRAIN CELLULOSE AND pH VALUE MAINTENANCE DURING SOLID-SUBSTRATE FERMENTATION[J].Chemistry & Industry of Forest Products,2004,24(4):81-86. |
| |
| Authors: | Abstract |
| |
| Abstract: | The production of low-cost cellulase enzyme is a key step in the development of an enzymatic-based process for conversion of lignocellulosic biomass to ethanol.Although abundant informations are available on cellulase production,little work has been examined on the effects of substrate pretreatment on pH value and cellulase formation during solid-substrate fermentation.This paper investigated pH value changes of substrate during fermentation of Trichoderma reesei on native and extruded substrates.After single-screw and twin-screw extrusion,cellulose crystallinity of spent grain was reduced slightly.Single extrusion fragmented and opened cellulose structure,while twin extrusion destroyed and disrupted the cell wall structure of spent grain as well as reduced particle size.Both extrusions favor micro-(organism) growth and enhance the production of cellulase.Maximal FPA activity of 182.8 IU/g cellulose was observed when spent grain by single extrusion was used as substrate. |
| |
| Keywords: | solid-substrate fermentation spent grain cellulase Trichoderma reesei |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《林产化学与工业》浏览原始摘要信息 |
