| 进境高粱种子中葡萄茎枯病菌的检疫鉴定 |
| |
| 引用本文： | 张 宇,许萍萍,吴 晶,杨 静,李 彬,吴翠萍.进境高粱种子中葡萄茎枯病菌的检疫鉴定[J].植物保护,2017,43(3):192-198. |
| |
| 作者姓名： | 张 宇 许萍萍 吴 晶 杨 静 李 彬 吴翠萍 |
| |
| 作者单位： | 1. 江苏出入境检验检疫局,南京,210001;2. 江苏省南京出入境检验检疫局,南京,210001 |
| |
| 基金项目： | 国家质量监督检验检疫总局科技计划(2012IK278);“十二五”国家科技支撑计划(2012BAK11B03) |
| |
| 摘 要： | 从进境的美国高粱样品中分离到一株与葡萄茎枯病菌Didymella glomerata相似的菌株4358-17。其菌落形态和显微特征均与葡萄茎枯病菌一致。多个位点(LSU、ITS、TUB2、ACT)序列比对显示菌株4358-17与GenBank登录号为KT389718、FJ427004、FJ427115、FJ426896的葡萄茎枯病菌相似性均达到100%。系统发育分析结果表明菌株4358-17与葡萄茎枯病菌株聚集在同一个分支上,支持率为99%。菌株4358-17接种高粱和小麦叶片,4d后接种部位出现明显症状。根据上述试验结果,将进境美国高粱样品中分离的菌株4358-17鉴定为葡萄茎枯病菌。
|
| 关 键 词： | 葡萄茎枯病菌 形态特征 系统发育分析 致病性测试 |
| 收稿时间： | 2016/8/1 0:00:00 |
| 修稿时间： | 2016/9/27 0:00:00 |
Identification of Didymella glomerata on imported Sorghum bicolor |
| |
| Zhang Yu,Xu Pingping,Wu Jing,Yang Jing,Li Bin,Wu Cuiping.Identification of Didymella glomerata on imported Sorghum bicolor[J].Plant Protection,2017,43(3):192-198. |
| |
| Authors: | Zhang Yu Xu Pingping Wu Jing Yang Jing Li Bin Wu Cuiping |
| |
| Institution: | 1. Jiangsu EntryExit Inspection and Quarantine Bureau, Nanjing 210001, China; 2. Nanjing EntryExit Inspection and Quarantine Bureau, Nanjing 210001, China |
| |
| Abstract: | The isolate 4358-17 was obtained from imported Sorghum bicolor from America. It was similar to Didymella glomerata in morphology. BLAST analysis indicated that LSU, ITS, TUB2 and ACT sequence showed 100% identity with the sequences with accession numbers of KT389718, FJ427004, FJ427115 and FJ426896, respectively. Phylogenetic analysis revealed that the isolate 4358-17 clustered within D.glomerata strains. Pathogenicity test showed that it could cause leaf spot on sorghum and wheat 4 d after inoculation. Based on all above results, the isolate 4358-17 was identified as D.glomerata. |
| |
| Keywords: | Didymella glomerata morphological characters phylogenetic analysis pathogenicity test |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《植物保护》浏览原始摘要信息 |
| 点击此处可从《植物保护》下载免费的PDF全文 |
|
