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钾肥与生物有机肥配施防治香蕉枯萎病效果初探
引用本文:秦艳梅,张志红,刘春卯.钾肥与生物有机肥配施防治香蕉枯萎病效果初探[J].植物保护,2017,43(3):65-69.
作者姓名:秦艳梅  张志红  刘春卯
作者单位:1. 河北省微生物研究所,保定,071051;2. 江西省科学院生物资源研究所,南昌,330096
基金项目:国家自然科学基金 (41001150)
摘    要:通过盆栽试验研究了生物有机肥与氯化钾和硫酸钾配施防治香蕉枯萎病的效果。试验结果表明,两种肥料配施促进了香蕉苗生长,降低了香蕉枯萎病病情指数,提高了防病效果,生物有机肥与KCl和K2SO4配施防病效果比单施生物有机肥分别高60%和90%。利用T-RFLP分析土壤细菌DNA多样性,生物有机肥与钾肥配施提高了细菌三个遗传多样性指数,增加了土壤中芽胞杆菌种类。FAME分析也发现生物有机肥以及与钾肥配施促进革兰氏阳性细菌和放线菌繁殖,抑制革兰氏阴性细菌和真菌生长。生物有机肥与钾肥配施,优势互补,改善了土壤微生物群落结构,有利于提高防病效果。

关 键 词:生物有机肥  香蕉枯萎病  钾肥  微生物群落结构
收稿时间:2016/6/26 0:00:00
修稿时间:2016/8/2 0:00:00

Effects of the combination of biofertilizer and potassium fertilizer on control of banana Fusarium wilt
Qin Yanmei,Zhang Zhihong,Liu Chunmao.Effects of the combination of biofertilizer and potassium fertilizer on control of banana Fusarium wilt[J].Plant Protection,2017,43(3):65-69.
Authors:Qin Yanmei  Zhang Zhihong  Liu Chunmao
Institution:1. Hebei Research Institute of Microbiology, Baoding 071051, China; 2. Biological Resources Institute, Jiangxi Academy of Sciences, Nanchang 330096, China
Abstract:The control effects of banana Fusarium wilt (Fusarium oxysporum f.sp.cubense, Foc) by biofertilizer (F),and its combination with potassium fertilizers of KCl and K2SO4 were studied. The soil microbial community diversity was investigated by FAME and TRFLP methods under greenhouse conditions. The results showed that biofertilizer and the combinations both promoted plant growth, reduced Fusarium wilt compared to the sterilized biofertilizer (control). The combinations of biofertilizer with KCl and K2SO4 increased control effect against wilt by 60% and 90% compared to biofertilizer treatment alone. The combinations improved soil Bacillus species and bacterial diversity indexes including richness, Shannon diversity and Shannon evenness. FAME results demonstrated that the combinations promoted Grampositive bacteria and actinomycetes, inhibited Gramnegative bacteria and fungi compared to control and biofertilizer treatment alone. The results suggested that the combinations of biofertilizer with potassium fertilizer could effectively control banana Fusarium wilt by enhancing the soil bacterial diversity and improving microbial community structure.
Keywords:biofertilizer  banana Fusarium wilt  potassium fertilizer  microbial community structure
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