| 矮壮素在大蒜试管苗生长和鳞茎形成上的应用研究 |
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| 引用本文： | 刘伟伟,刘宇峰,宋淑敏,王晓楠,潘静.矮壮素在大蒜试管苗生长和鳞茎形成上的应用研究[J].安徽农业科学,2013(28):11291-11292,11316. |
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| 作者姓名： | 刘伟伟 刘宇峰 宋淑敏 王晓楠 潘静 |
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| 作者单位： | 黑龙江省科学院大庆分院,黑龙江大庆,163319;黑龙江省科学院大庆分院,黑龙江大庆,163319;黑龙江省科学院大庆分院,黑龙江大庆,163319;黑龙江省科学院大庆分院,黑龙江大庆,163319;黑龙江省科学院大庆分院,黑龙江大庆,163319 |
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| 基金项目： | 黑龙江省财政厅基本业务专项基金项目(DJZ2011-04) |
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| 摘 要： | 目的]研究矮壮素在大蒜试管苗生长和鳞茎形成上的应用。方法]用大蒜幼嫩气生鳞茎为外植体培养的试管苗为材料，以MS为基本培养基，系统研究不同浓度矮壮素（CCC）处理对大蒜试管苗生长和鳞茎形成的影响。结果]在MS培养基中加入0．4mg／L的矮壮素能有效使大蒜试管苗增高、增粗，叶色加深。在试管鳞茎诱导条件下，矮壮素对大蒜试管鳞茎形成和膨大作用显著（P〈0．05），0．5mg／L矮壮素处理时鳞茎形成率为793％，鳞茎总诱导率为96．67％。当矮壮素浓度为0．6mg／L时，试管鳞茎鲜重、平均直径都明显增加。结论]试验研究了矮壮素在大蒜试管苗生长和鳞茎形成上的应用，为大蒜的栽培驯化提供了理论依据。
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| 关 键 词： | 大蒜(Allium sativum L.) 矮壮素 试管鳞茎 |
Effect of CCC on Explants Growth and Bulb Formation of Garlic |
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| Institution: | LIU Wei-wei et al ( Daqing Branch of Heilongjiang Academy of Sciences, Daqing, Heilongjiang 163319) |
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| Abstract: | Objective ] To study effect of CCC on explants growth and bulb formation of garlic. Method] Using rachis of garlic as explants and taking MS as basic culture medium, effects of different concentration CCC on explants growth and bulb formation of garlic were studied. Result] The results showed that the growth of garlic explants was improved by the application of 0.4 mg/L CCC, the growth of stems and leaves was thicker and leaf color was greener. Under the bulblet inductive condition, CCC has a significant effect （ P 〈 0.05 ） on formation of garlic bulb and enlargement in vitro. The formation rate of bulb with 0.5 mg/L CCC treatment was 793% and total induction rate of bulb was 96.67%. 0.6 mg/L CCC treatment promoted increasing of bulb fresh weight and percentage of abnormal bulb. Conclusion ] The effect of CCC on explants growth and bulb formation of garlic was studied, which provides theoretical basis for cultivation of garlic. |
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| Keywords: | Garlic CCC Bulblet |
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