| 番茄果实高质量总RNA的提取富集及RT-PCR检测 |
| |
| 引用本文： | 迟丽红,马远征,刘海平,田慧琴,罗云波,朱本忠.番茄果实高质量总RNA的提取富集及RT-PCR检测[J].农机化研究,2011,33(3). |
| |
| 作者姓名： | 迟丽红 马远征 刘海平 田慧琴 罗云波 朱本忠 |
| |
| 作者单位： | 中国农业大学食品科学与营养工程学院,北京,100083 |
| |
| 摘 要： | 以番茄果实为材料,比较了3种在果实中提取RNA的方法,针对番茄果实多糖的特点,利用无水乙醇和异丙醇联合沉淀的方法除去多糖,改良了Trizol法.为了检验含量比较低的miRNA,利用Licl沉淀的方法进行富集.结果表明:改良Trizol法能够有效地去除多糖,提取到的RNA 28S rRNA亮度约为18S rRNA的2倍,A260与A280比值为1.84,A260与A230比值为1.92,RNA产率为350.4 μg/g,说明利用改良Trizol法从番茄果实中提取的RNA质量好、完整性好.由此法所得的RNA可直接用于cDNA文库构建等分子生物学实验.
|
| 关 键 词： | 番茄 改良Trizol法 |
Extraction of High Quality Total RNA from Tomato Fruit and RT- PCR Test |
| |
| Chi Lihong,Ma Yuanzheng,Liu Haiping,Tian Huiqin,Luo Yunbo,Zhu Benzhong.Extraction of High Quality Total RNA from Tomato Fruit and RT- PCR Test[J].Journal of Agricultural Mechanization Research,2011,33(3). |
| |
| Authors: | Chi Lihong Ma Yuanzheng Liu Haiping Tian Huiqin Luo Yunbo Zhu Benzhong |
| |
| Institution: | Chi Lihong,Ma Yuanzheng,Liu Haiping,Tian Huiqin,Luo Yunbo,Zhu Benzhong(Collage of Food Science and Nutritional Engineering,China Agriculture University,Beijing 100083,China) |
| |
| Abstract: | Trizol method,guanidine thiocyanate method and perchlorate method,were employed to extract total RNA in fruit of tomato.And Trizol method was accordingly modified in extracting the total RNA in tomato by integrating a polysaccharide-removing step in correspondence with the fact that tomato fruit was rich in polysaccharides and polyphenol.Due to the low content of miRNA,we were using Licl precipitation method to enrichment it.The results showed that the modified Trizol method was capable of efficiently remov... |
| |
| Keywords: | miRNA |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
