| 一株产异甘露聚糖酶菌株发酵条件的优化 |
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| 引用本文： | 王法云,竹磊,田春华,朱海华.一株产异甘露聚糖酶菌株发酵条件的优化[J].安徽农业科学,2010,38(33):18661-18663. |
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| 作者姓名： | 王法云 竹磊 田春华 朱海华 |
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| 作者单位： | 河南省食品质量安全控制工程技术研究中心,河南郑州,450002;河南省商业科学研究所有限责任公司,河南郑州,450002 |
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| 基金项目： | 河南省科学院专项资金项目 |
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| 摘 要： | 目的]确定SKS4的最佳发酵产酶条件。方法]以SKS4为试材,通过单因素试验和正交试验对SKS4的最佳发酵条件进行了优化。结果]在酵母多糖浓度为5.0%,氮源浓度为0.4%,培养基初始pH值为7.0,接种量为4.0%,装液量为40 ml/500 ml,培养温度为37℃时,蜡样芽孢杆菌SKS4产生的异甘露聚糖酶活力达2 185 U/ml。结论]建立了最佳的SKS4产酶发酵条件,获得了较高的酶活。
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| 关 键 词： | 异甘露聚糖酶 蜡样芽孢杆菌 优化 正交试验 |
The Ferment Condition's Optimization of a Bacterial Strain Which Can Producing Isomerism Mannanase |
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| Institution: | WANG Fa-yun et al ( Henan Engineering Research Center of Food Quality Safety Control, Zhengzhou, Henan 450002) |
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| Abstract: | Objective] The purpose of the study was to identify the optimum conditions for produce mannanase of SKS4.Method] By means of monofactorial experiment and orthogonal optimization test,identified the optimum conditions for produce mannanase,with SKS4 as tested materials.Result] The concentration of isomerism mannanase which extracted by ourself is 5.0%,nitrogen concentration is 0.4%,the initial pH of culture medium is 7.0,inoculum concentration is 4.0%,liquid volume is 40 ml/500 ml,temperature of fermentation is 37 ℃,the enzyme activity of producing by isomerism mannanase can be reached 2 185 U/ml.Conclusion] The excellent enzyme activity can be obtained with the optimum culticivation method. |
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| Keywords: | Isomerism mannanase Bacillus cereus Optimization Orthogonal design |
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