Use of multiparental inbred populations to determine allelic relationships of molecular markers |
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| Authors: | J Enjalbert C Boeuf H Belcram P Leroy |
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| Institution: | INRA, INAPG, UPS, Station de Génétique Végétale, Ferme du Moulon, F-91190 Gif sur Yvette, France;INRA, Station d'Amélioration des Plantes, 234 Avenue du Brézet, F-63039 Clermont-Ferrand Cedex 2, France |
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| Abstract: | The assessment of polymorphism exhibited by molecular markers is an arduous but essential task that facilitates the use of molecular tools by breeders and geneticists. For that purpose, the value of a wheat composite population was assessed for characterizing the diversity of restriction fragment length polymorphism (RFLP) markers developed by INRA-Génoblé. The polymorphism of 13 genomic probes was measured over a set of 80 inbred lines randomly extracted by single-seed descent from a composite-cross of 16 wheat lines. The 13 probéenzyme combinations revealed 27 loci with codominant polymorphism. As many bands were so far unmapped, the segregational analysis of the progenies appeared very suitable for complex patterns, both in determining allelic relationships and in revealing linkage between loci. Allelic diversity, band sizes and chromosomal location assessed from nullisomic-tetrasomic lines are given for the 27 loci. |
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| Keywords: | Triticum aestivum chromosomal location multiparental cross polymorphism RFLP markers |
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