| 表达传染性喉气管炎病毒gB基因重组鸡痘病毒疫苗免疫持续期试验 |
| |
| 引用本文： | 王云峰,智海东,王玫,彭金美,仇华吉,周艳君,田志军,张绍杰,童光志.表达传染性喉气管炎病毒gB基因重组鸡痘病毒疫苗免疫持续期试验[J].中国预防兽医学报,2004,26(2):131-135. |
| |
| 作者姓名： | 王云峰 智海东 王玫 彭金美 仇华吉 周艳君 田志军 张绍杰 童光志 |
| |
| 作者单位： | 中国农业科学院,哈尔滨兽医研究所,兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划)，科技部农业科技成果转化基金 |
| |
| 摘 要： | 将200003批疫苗免疫4周龄SPF鸡,免疫后的7、14、21、30、60、90、120、150、180和225 d分别采血,分离血清,采用ELISA方法检测血清抗FPV抗体,结果表明重组疫苗免疫后14 d,免疫鸡血清抗体已经全部阳转,免疫后的21 d血清抗FPV的抗体出现峰值,此后便开始下降,到免疫后的6个月抗体水平已经接近阴性对照的水平.用ILTV WG株和FPV 102株强毒进行的攻毒保护试验与血清检测的结果基本一致,在免疫后的5个月内可以使免疫鸡获得100%(10/10)保护,免疫后的6个月对ILT和FP的免疫保护分别为1/7和2/10,此时需要对鸡群实施二次免疫.其他5批疫苗(200001、200002、200101、200102和200103)免疫SPF鸡后5个月用ILTV WG株和FPV 102株攻击也获得了完全保护.
|
| 关 键 词： | 传染性喉气管炎 鸡痘 重组鸡痘病毒 免疫持续期 |
| 文章编号： | 1008-0589(2004)02-0131-05 |
| 修稿时间： | 2003年5月23日 |
Duration of recombinant fowlpox virus vaccine expressing gB of Infectious laryngotracheitis virus |
| |
| WANG Yun-feng,ZHI Hai-dong,WANG Mei,PENG Jin-mei,QIU Hua-ji,ZHOU Yan-jun,TIAN Zhi-jun,ZHANG Shao-ji,TONG Guang-zhi h Institute of Chinese Academy of Agricultural Sciences,Harbin, China.Duration of recombinant fowlpox virus vaccine expressing gB of Infectious laryngotracheitis virus[J].Chinese Journal of Preventive Veterinary Medicine,2004,26(2):131-135. |
| |
| Authors: | WANG Yun-feng ZHI Hai-dong WANG Mei PENG Jin-mei QIU Hua-ji ZHOU Yan-jun TIAN Zhi-jun ZHANG Shao-ji TONG Guang-zhi h Institute of Chinese Academy of Agricultural Sciences Harbin China |
| |
| Institution: | WANG Yun-feng,ZHI Hai-dong,WANG Mei,PENG Jin-mei,QIU Hua-ji,ZHOU Yan-jun,TIAN Zhi-jun,ZHANG Shao-ji,TONG Guang-zhi~* h Institute of Chinese Academy of Agricultural Sciences,Harbin,150001 China) |
| |
| Abstract: | Four-week-old SPF chickens were inoculated with fowlpox virus vaccine expressing gB of infectious laryngotracheitis virus (lot 200003). On the day of 7, 14, 21, 30, 60, 90, 120, 150, 180 and 225 post inoculation(PI), blood sample were taken, and the titer of antibody against FPV were detected by ELISA. All of the chickens' antibody were positive at 14th day, and reached peak on 21st day PI, then declined gradually. At the 6th month, it dropped to the negative value. The challenge results of virulent ILTV and FPV is the same as changes of FPV antibody. The vaccine could offer 100% protection in 5 months, but only 1/7 and 2/10 chickens were protected when challenged at the 6th month PI. The vaccine from other five lots had also obtained the same protection results as described. |
| |
| Keywords: | infectious laryngotracheitis virus fowlpox virus recombinant fowlpox virus duration |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
