| 植物转座元件及其分子标记的研究进展 |
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| 引用本文： | 代红艳,张志宏.植物转座元件及其分子标记的研究进展[J].农业生物技术学报,2006,14(3):434-439. |
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| 作者姓名： | 代红艳 张志宏 |
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| 作者单位： | 沈阳农业大学园艺学院,沈阳,110161 |
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| 基金项目： | 国家自然科学基金;辽宁省高等学校优秀人才支持计划;沈阳农业大学校科研和教改项目 |
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| 摘 要： | 转座元件是基因组中可移动的DNA分子，在真核生物的基因和基因组进化中起着重要的作用。植物的转座元件分为反转录转座子和DNA转座子两大类，其中，反转录转座子又分为长末端重复序列(LTR)反转录转座子和非LTR反转录转座子两个亚类，而DNA转座子分为自主元件、非自主元件和微型反向重复转座元件(MITE)三种类型。基于植物转座元件的分子标记目前主要有序列特异扩增多态性(S-SAP)、MITE显示、转座子显示(TD)、MITE位点间多态性(IMP)、反转录转座子位点(IRAP)、反转录转座子-微卫星扩增多态性(REMAP)和基于反转录转座子插入多态性(RBIP)。综述了这些分子标记技术的原理及其在生物遗传多样性与系统进化分析、基因作图、品种鉴定等方面的应用。
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| 关 键 词： | 转座元件 分子标记 遗传分析 |
| 文章编号： | 1006-1304（2006）03-0434-06 |
| 收稿时间： | 2005-3-30 |
| 修稿时间： | 2005-4-25 |
Progress on Plant Transposable Elements and Their Molecular Markers |
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| DAI Hong-yan,ZHANG Zhi-hong.Progress on Plant Transposable Elements and Their Molecular Markers[J].Journal of Agricultural Biotechnology,2006,14(3):434-439. |
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| Authors: | DAI Hong-yan ZHANG Zhi-hong |
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| Institution: | College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China |
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| Abstract: | Transposable elements (TEs), which are DNA segments moving within genomes, play a major role in gene and genome evolution of eukaryotes. Plant transposable elements are classified into two major groups: class Ⅰelements or retrotransposons and class Ⅱelements or DNA transposons. Retrotransposons are divided into two principal groups, the long terminal repeat (LTR) and the non-LTR retrotransposons, while DNA transposons are divided into three types, the autonomous elements, non-autonomous elements and miniature inverted-repeat transposable elements (MITEs). The molecular markers based on plant TEs，including sequence-specific amplification polymorphism (S-SAP), MITE display, transposon display (TD), inter-MITE polymorphism (IMP), inter-retrotransposon amplified polymorphism (IRAP), retrotransposon-microsatellite amplified polymorphism (REMAP) and retrotransposon-based insertion polymorphism (RBIP), were developed. In this review, the principles and applications of above-mentioned markers in genetic biodiversity and phylogeny analyses, gene mapping, and cultivar certification, were summarized. |
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| Keywords: | transposable element molecular marker genetic analysis |
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