| 卡瓦胡椒及胡椒的AFLP分子标记探讨 |
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| 引用本文： | 施江,辛莉,杨彦,郑学勤.卡瓦胡椒及胡椒的AFLP分子标记探讨[J].农业科学与技术,2007,8(3):5-11. |
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| 作者姓名： | 施江 辛莉 杨彦 郑学勤 |
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| 作者单位： | [1]河南科技大学农学院,河南洛阳471003 [2]北京鼎国生物技术有限责任公司,北京100088 [3]热带作物生物技术国家重点实验室,海南海口571101 |
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| 基金项目： | 国家科技攻关项目;河南科技大学校科研和教改项目 |
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| 摘 要： | 扩增片段长度多态性（AFLP）技术是一项新的分子标记技术，是基于PCR技术扩增基因组DNA限制性片段，基因组DNA先用限制性内切酶切割，然后将双链接头连接到DNA片段的末端，接头序列和相邻的限制性位点序列作为引物结合位点。笔者采用AFLP分子标记技术探讨了卡瓦胡椒与胡椒及胡椒属其他几个野生种的亲缘关系。
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| 关 键 词： | Piper methysticum Pepper Amplified fragment length pslymorphism 卡瓦胡椒 AFLP 分子标记 Pepper Piper Molecular Markers fingerprint construction molecular identification graft provided basis family related clustered single groups silver staining genetic |
| 修稿时间： | 2007年11月7日 |
Discussion on AFLP Molecular Markers in Piper methysticum and Pepper |
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| SHI Jiang,XIN Li,YANG Yan,ZHANG Xue-qin.Discussion on AFLP Molecular Markers in Piper methysticum and Pepper[J].Agricultural Science ＆ Technology,2007,8(3):5-11. |
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| Authors: | SHI Jiang XIN Li YANG Yan ZHANG Xue-qin |
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| Abstract: | The aim of the research was to discuss the genetic relationships between Pier methysticum.Pepper and other wild species in Pepper genus.DNA Was extracted from leaves which belonged tp 28 germplasras including 6 materials of P. methysticum,21 ma erials of cultivated and wild Pepper,1 material of Peperomia pellucida belonged to different genus.Premiers with good band-type and high polymorphism and resolution were selected from 64 pairs of Drimers for AFLP amplification and the clustering analysis was conducted with MVSP3.13f software.191 bands were amplified by 4 pairs of premiers,189 of which had polymorphism,being 98.6%. 28 germplasms were classified into 6 different groups al the genetic similarity coefficient of 0.52 by silver staining AFLP,in which 6 materials of Piper methysticum were clustered into a single group,indicating that P. methysticum belonged to Pepper family of Pepper genus but were distantly related to the others.The research provided the basis for selecting rootstocks for P. methysticum graft,molecular identification of P. methysticum and the fingerprint construction of P.methysticum. |
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| Keywords: | Piper methysticum Pepper Amplified fragment length pslymorphism |
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