| 一种新的鉴定水稻初级三体的方法 |
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| 引用本文： | 陈红,秦瑞珍.一种新的鉴定水稻初级三体的方法[J].作物学报,2001,27(6):923-928. |
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| 作者姓名： | 陈红 秦瑞珍 |
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| 作者单位： | 中国农业科学院作物育种栽培研究所, 农业部遗传育种重点实验室, ，北京 100081 |
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| 基金项目： | national 863 plan(Z16-02-01-03). Receive d on: Studies on the new way to create primary trisomics of rice. |
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| 摘 要： | 水稻初级三体在水稻遗传育种中起着重要的作用.其归属鉴定通常用Shastry(1960)的粗线期核型分析方法,但这种方法在实际操作中有一定的难度,不易得到分散好的粗线期染色体分裂相.本研究首次尝试利用三体株和正常株同源染色体间DNA含量的差异,用分子标记的手段将量的差异转为自显影带的差异原理去鉴定三体,发现此鉴定结果与粗线
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| 关 键 词： | 水稻 初级三体 粗线期核型分析 分子标记 |
| 收稿时间： | 2001-03-01 |
| 修稿时间： | 2001年3月1日 |
A New Method for the Identifying Primary Trisomics of Rice |
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| CHEN Hong,QIN Rui,Zhen.A New Method for the Identifying Primary Trisomics of Rice[J].Acta Agronomica Sinica,2001,27(6):923-928. |
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| Authors: | CHEN Hong QIN Rui Zhen |
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| Abstract: | The primary trisomics of rice play an important role in study of genet ics and breeding of rice. Researchers always identify primary trisomics by the k aryotype analysis of pachtene firstly used by Shastry(1960). But this method is not easy to obtain good result because it needs superb skill. In this study, a n ew way to identify primary trisomics was used. It makes use of the difference in the quantity of homologous DNA between primary trisomics and diploid rice and d emonstrated the difference with molecular marker. The identification result by m olecular marker is the same as that by karyotype analysis. This method is credib le and quick, so it will be a new and useful means to identify primary trisomics |
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| Keywords: | Rice Primary trisomics Karyotype anal ysis of pach Molecular marker |
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