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脐橙果实发育过程中有机酸代谢相关酶的研究
引用本文:龚荣高,吕秀兰,张光伦,胡世学.脐橙果实发育过程中有机酸代谢相关酶的研究[J].四川农业大学学报,2006,24(4):402-404.
作者姓名:龚荣高  吕秀兰  张光伦  胡世学
作者单位:1. 四川农业大学,林学园艺学院,四川,雅安,625014
2. 重庆市奉节县农业局,重庆,奉节,404600
基金项目:四川省农业厅重点支持项目;四川省教育厅资助项目
摘    要:以罗伯逊脐橙为试材,研究了脐橙果实有机酸代谢相关酶的活性变化动态,及其与果实有机酸积累关系,旨在探索影响脐橙果实有机酸含量的重要酶。结果表明:果实有机酸的积累期,CS、PEPC活性随有机酸积累而增大,并呈显著正相关;有机酸降解期,IDH的活性与有机酸含量呈极显著负相关;而MDH和ACO对酸含量的影响小。因而可认为PEPC和CS是影响果实有机酸合成的重要酶,而影响有机酸分解的重要酶则是IDH。

关 键 词:脐橙  有机酸积累  代谢相关酶
文章编号:1000-2650(2006)04-0402-03
收稿时间:2006-09-12
修稿时间:2006年9月12日

Study on the Organic Acid-metabolizing Enzymes in Robertson Navel Orange during the Fruit Development
GONG Rong-gao,Lü Xiu-lan,ZHANG Guang-lun,HU Shi-xue.Study on the Organic Acid-metabolizing Enzymes in Robertson Navel Orange during the Fruit Development[J].Journal of Sichuan Agricultural University,2006,24(4):402-404.
Authors:GONG Rong-gao  Lü Xiu-lan  ZHANG Guang-lun  HU Shi-xue
Institution:1.College of Forestry and Horticulture, Sichuan Agricultural University, Yaan 625014, Sichuan, China; 2. Agricultural Bureau of Fengjie County, Chongqing 404606, China
Abstract:'Robertson Navel'orange(Citrus sinesis Osb.)fruits were used to study organic acid-metabolizing enzymes during the fruit development,especially the changes in activities of citrate synthase(CS),phosphoenolpyruvatecarboxylase(PEPC),cis-aconitase(ACO),isocitrate dehydrogenase(IDH)and malate dehydrogenase(MDH),in order to find out the key enzymes which influences the organic acid content in navel fruit.The results show that CS and PEPC play an important role in organic acid accumulation during the early stage of the fruit development,while IDH is the key enzyme that cleave citrate from 120 days after full bloom to the fruit ripening.However,MDH and CAO are not the key enzymes that influence the organic acid accumulation.
Keywords:navel orange  organic acid accumulation  metabolizing enzyme
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