Analysis of the electrophoretic karyotype ofFlammulina velutipes |
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| Authors: | Doolyi Kim Yutaka Tamai Tomonori Azuma Akira Harada Akira Ando Yoh Sakuma Kiyoshi Miura |
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| Institution: | (1) Laboratory of Forest Pathology, Division of Forest Biology, Forestry Research Institute, 207 Chungryang 2-dong, 130-012 Dongdaemoon-gu, Seoul, Korea;(2) Division of Environmental Resources, Graduate School of Agriculture, Hokkaido University, N9-W9 Kita-ku, 060-8589 Sapporo, Japan;(3) Forest Products Research Institute, 1-10 Nishi-kagura, 071-0181 Asahikawa, Japan;(4) Japan International Research Center for Agricultural Sciences, 1-2 Owashi, 305-8686 Tsukuba, Japan |
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| Abstract: | The karyotype ofFlammulina velutipes (Curt.: Fr.) Sing. was analyzed electrophoretically using contourclamped homogeneous electric field gel electrophoresis and hybridization with DNA probes. The chromosomal DNA from the monokaryon (Fv-4K) and the dikaryon (Fv-4) were resolved into six and eight bands, respectively. The sizes of the chromosomes ranged from 1.9 to 6.0 megabase (Mb) pairs. Each of the separated bands of chromosomal DNA was identified by use of five cloned probes. The number of these chromosomes was estimated to be 6 and 12, respectively; and the size of the entire genome was estimated to be about 20.1 and 38.6Mb, respectively. From a comparison of the hybridization patterns, the existence of allelic chromosomes of different sizes was deduced in the Fv-4 strain. |
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| Keywords: | Flammulina velutipes Electrophoretic karyotype Chromosome CHEF |
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