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| 棉花黄萎病内生拮抗细菌的分离及LC-04菌株的鉴定 | |
| 引用本文： | 王世英,杜红方,李术娜,张元亮,朱宝成.棉花黄萎病内生拮抗细菌的分离及LC-04菌株的鉴定[J].湖北农业科学,2009,48(2). |
| 作者姓名： | 王世英 杜红方 李术娜 张元亮 朱宝成 |
| 作者单位： | 1. 河北农业大学生命科学学院,河北,保定,071001 2. 河北农业大学生命科学学院,河北,保定,071001;河北大学生命科学学院,河北,保定,071002 3. 河北大学生命科学学院,河北,保定,071002 |
| 摘 要： | 从棉花根、茎、叶部组织中分离到19株对棉花黄萎病菌大丽轮枝菌有枯抗作用的细菌,其中抑菌圈直径大于20mm的菌株1株.直径10～20mm之间的菌株5株,直径小于10mm的菌株13株.分离自棉花叶部组织的LC-04菌株对大丽轮枝菌V-190菌株生长的抑制作用最强.通过时LC-04菌株菌体形态、菌落特征的观察和一系列生理生化试验分析,该菌株鉴定为类芽孢杆菌属细菌. |
| 关 键 词： | 棉花黄萎病 大丽轮枝菌 拮抗细菌 分离 鉴定 |
Isolation of Endophytic Antagonistic Bacterial Strains in Cotton against Verticillium dahliae |
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| WANG Shi-ying,DU Hong-fang,LI Shu-na,ZhANG Yuan-liang,ZHU Bao-cheng.Isolation of Endophytic Antagonistic Bacterial Strains in Cotton against Verticillium dahliae[J].Hubei Agricultural Sciences,2009,48(2). | |
| Authors: | WANG Shi-ying DU Hong-fang LI Shu-na ZhANG Yuan-liang ZHU Bao-cheng |
| Institution: | 1.College of Life Science;Agricultural University of Hebei;Baoding 071001;Hebei;China;2.College of Life Science;Hebei University;Baoding 071002;China |
| Abstract: | The endophytic bacteria strains were isolated from the different cotton tissue, such as stems, leaves, roots which were treated with surface sterilization technology after washing. 19 strains which had antagonistic activities against Verticillium dahliae Kleb were obtained. The results showed that there was one strain whose inhibition zone diameter was above 20mm , five strains between 10mm and 20mm, thirteen strains in 10mm. The strain LC-04 isolated from the cotton leaves had a strong antagonistic activit... |
| Keywords: | cotton Verticillium wilt Verticillium dahliae antagonistic bacteria isolation identification |
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