| 蜡蚧轮枝菌的碳·氮营养源研究 |
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| 引用本文： | 刘春来,王爽,李新民,刘兴龙,夏吉星,杨帆,马玲.蜡蚧轮枝菌的碳·氮营养源研究[J].安徽农业科学,2013(32):12595-12596,12599. |
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| 作者姓名： | 刘春来 王爽 李新民 刘兴龙 夏吉星 杨帆 马玲 |
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| 作者单位： | 东北林业大学林学院,黑龙江哈尔滨150040;黑龙江省农业科学院植物保护研究所,黑龙江哈尔滨150086;黑龙江省农业科学院植物保护研究所,黑龙江哈尔滨,150086;东北林业大学林学院,黑龙江哈尔滨,150040 |
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| 基金项目： | 国家国际科技合作专项项目（2012DFR30810）. |
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| 摘 要： | 目的]筛选影响蜡蚧轮枝菌生长的营养因子，为该菌的开发生产提供科学依据。方法]以固体基础培养基1L水中20g葡萄糖的含碳量（8．00g／L）、2g蛋白胨的含氮量（0．29g/L）及少量无机盐为标准，用蔗糖等碳源及酵母膏等氮源分别取代基础培养基中的葡萄糖和蛋白胨。研究了固体培养基中不同碳、氮源对6株蜡蚧轮枝菌菌丝生长和产孢的影响。结果]葡萄糖有利于V2、V4、V5和V6菌株的菌丝生长和产孢；可溶性淀粉为碳源时，V1和V3菌株的生物量和产孢量均很高；有机氮源酵母膏可大大提高各菌株的生物量及产孢量。结论]葡萄糖与酵母膏组合是适合于多数供试蜡蚧轮枝菌菌株菌丝生长和产孢的碳氮源。．
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| 关 键 词： | 蜡蚧轮枝菌 碳源 氮源 生物量 产孢量 |
Research on Carbon and Nitrogen Sources of Verticillium lecanii Cultured on Solid Matrix |
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| Institution: | LIU Chun-lai et al (Plant Protection Institute of Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang 150086) |
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| Abstract: | Objective ] The study aimed to select nutritional factors of effeeting growth on Verticillium lecanii. Method] Based on a defined bas-al medium composed of 20g glucose （equivalent to 8.0 g/L carbon）, 2 g peptone （equivalent to 0.29 g/L nitrogen）, and small amount of inor-ganic salt in 1 liter of water, the six VerticiUium lecanii strains were cultured on solid agar media which glucose or peptone in basal medium were substituted by various carbon sources （sucrose etc） or nitrogen sources （yeast extract etc） at the same C or N concentrations of basal medium re- spectively. The effect of carbon or nitrogen sources on mycelial growth and sporulation of strains was evaluated via measurement of colony growth and spore yield. Resuh] The results showed that glucose was more suitable for the myeelial growth and sporulation of V4 、V5、V6 strains. Strains of V1 and V3 produced a high biomass and high concentrations of spores with soluble amylum; the biomass and the sporulation quantity of all six strains could be produced in large quantity with yeast extract of organic nitrogen source. Conclusion] The combination of glucose and yeast ex-tract was suitable for mycelial growth and sporulation, of the six V. lecanii strains. |
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| Keywords: | Verticillium lecanii Carbon source Nitrogen source Biomass Sporulation |
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