| 氯氰菊酯与p53-DNA相互作用的光谱研究 |
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| 引用本文： | 卜晓阳,杨小弟,陈小勇.氯氰菊酯与p53-DNA相互作用的光谱研究[J].安徽农业科学,2013(36):13895-13896,13913. |
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| 作者姓名： | 卜晓阳 杨小弟 陈小勇 |
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| 作者单位： | 皖南医学院药学院,安徽芜湖,241002;南京师范大学化学与材料科学学院,江苏南京,210097;芜湖市第二人民医院骨科,安徽芜湖,241000 |
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| 基金项目： | 皖南医学院中青年科研基金项目（WK201315）;国家自然科学基金（20902048）. |
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| 摘 要： | 采用紫外、荧光分光光度法研究了农药氯氰菊酯与人类肿瘤抑制基因p53-DNA之间的相互作用.实验出现紫外减色、荧光猝灭等结果表明氯氰菊酯可能是以嵌插模式与p53-DNA结合作用;紫外变温实验得出p53-DNA熔点温度略有升高等结果则进一步证实氯氰菊酯是部分嵌插入DNA的沟区,影响了DNA的双螺旋结构的稳定性.
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| 关 键 词： | 光谱法 氯氰菊酯 p53-DNA 相互作用 |
Study on the Interaction of Cypermethrin with p53-DNA by Means of Spectrometry |
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| Institution: | BU Xiao-yang( 1.School of Pharmacy, Wannan Medical College, Wuhu, Anhui 241002;) |
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| Abstract: | The interaction of Cypermethrin with p53-DNA was studied by means of UV,fluorescence and CD spectrophotometry.The UV experiments,fluorescence quenching experiments showed an intercalation between Cypermethrin and DNA; Besides,melting experiments also confirmed that Cypermethrin interacts with DNA via partly interaction binding,thus affecting the double helix structure of DNA. |
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| Keywords: | Spectrometry Cypermethrin p53-DNA Interaction effect |
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