High incidence of single nucleotide polymorphisms in the prion protein gene of native Brazilian Caracu cattle |
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| Authors: | WA Kues RD Ollhoff JW Carnwath FP de Souza HMF Madeira & H Niemann |
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| Institution: | Department of Biotechnology, Institute for Animal Breeding (FAL), Mariensee, Germany; Pontifícia Universidade Católica do Paraná- PUCPR, São Josédos Pinhais, Paraná, Brasil |
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| Abstract: | Different alleles of the human and ovine prion protein gene correlate with a varying susceptibility to transmissible spongiform encephalopathies. However, the pathogenic implications of specific polymorphisms in the bovine prion protein gene (PRNP) are only poorly understood. Previous studies on the bovine PRNP gene investigated common European and North American cattle breeds. As a consequence of decades of intensive breeding for specific traits, these modern breeds represent only a small fraction of the bovine gene pool. In this study, we analysed PRNP polymorphisms in the native Brazilian Caracu breed, which developed in geographical isolation since the 16th century. A total of 10 single nucleotide polymorphisms (SNPs) were discovered in the coding region of the Caracu PRNP gene. Eight of the SNPs occurred at high frequencies in Caracu cattle (variant allele frequencies = 0.10–0.76), but were absent or only rarely observed in European and North American breeds. One of the Caracu SNPs was associated with an amino acid exchange from serine to asparagine (f = 0.17). This SNP was not detected in Holstein–Friesian, Simmental and German Gelbvieh and was only rarely detected in beef cattle (f = 0.01). We found 17 haplotypes for PRNP in the Caracu breed. |
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| Keywords: | bovine spongiform encephalopathy genomic sequencing haplotype prion protein single nucleotide polymorphisms |
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