| 茉莉酸甲酯诱导的苹果悬浮细胞的防卫响应 |
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| 引用本文： | 刘志,张华磊,谢兴斌,束怀瑞,郝玉金.茉莉酸甲酯诱导的苹果悬浮细胞的防卫响应[J].园艺学报,2009,36(2):171-178. |
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| 作者姓名： | 刘志 张华磊 谢兴斌 束怀瑞 郝玉金 |
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| 作者单位： | （山东农业大学作物生物学国家重点实验室，山东泰安 271018；山东农业大学园艺科学与工程学院，山东泰安 271018;辽宁省果树科学研究所, 辽宁熊岳 115009） |
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| 基金项目： | 国家高技术研究发展计划(863计划)，教育部新世纪优秀人才支持计划，山东省良种工程项目，辽宁省重点实验室专项资金项目 |
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| 摘 要： | 以苹果悬浮细胞为试材，以硝普钠(SNP)为一氧化氮(NO)供体，L-NAA(Nx-nitro-L- arginine)为一氧化氮合酶(NOS)抑制剂，PTIO(2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl- 3-oxide)为NO清除剂，研究了茉莉酸甲酯(MeJA)处理苹果悬浮细胞诱发NO和H2O2信号分子及其对PAL酶活性、植保素和丙二醛(MDA)的影响。结果表明，MeJA处理能明显诱发NO生成，在处理6 h时NO生成量达到峰值，而L-NAA和PTIO则能够抑制NO生成。另外，MeJA处理也显著提高了细胞内的H2O2水平，而SNP单独处理不能提高H2O2水平。在各种处理中，PAL活性以及植保素和MDA含量均表现出先升后降趋势，分别在4 h或6 h时达到峰值。本研究结果揭示了MeJA可以激发NO生成，而细胞内H2O2水平的提高则是MeJA和NO协同作用的结果，植保素和MDA含量以及PAL活性的提高主要受NO的诱导，但也存在其它诱导途径。
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| 关 键 词： | 苹果 悬浮细胞 激发子 茉莉酸甲酯 一氧化氮 PAL 植保素 |
| 收稿时间： | 2008-10-14 |
| 修稿时间： | 2009-1-19 |
Methyl Jasmonate-induced Defense Responses of Apple Suspension Cells |
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| LIU Zhi,ZHANG Hua-lei,XIE Xing-bin,SHU Huai-rui,HAO Yu-jin.Methyl Jasmonate-induced Defense Responses of Apple Suspension Cells[J].Acta Horticulturae Sinica,2009,36(2):171-178. |
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| Authors: | LIU Zhi ZHANG Hua-lei XIE Xing-bin SHU Huai-rui HAO Yu-jin |
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| Institution: | (State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, Shandong 271018, China; College of Horticultural Science and Engineering, Shandong Agricultural University, Taian, Shandong 271018, China; Liaoning Institute of Pomology, Xiongyue, Liaoning 115009, China) |
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| Abstract: | Meythyl Jasmonate (MeJA) is an elicitor for defensive responses of plant to pathogens, while NO and H2O2 are signal molecules related to defensive responses. In this study, MeJA-induced defense responses of apple suspension cells were monitored in NO release, H2O2 accumulation, PAL activity and MDA content, with sodium nitroprusside (SNP) as a NO donor, L-NAA (Nx-nitro-L-arginine) as NOS inhibitor and PTIO (2-phenyl-4,4,5,5-tetramethyl- imidazoline-1-oxyl-3-oxide) as NO scavengers. The results showed that apple suspension cells constantly produced NO and reached a peak release at 6h when exposed to MeJA treatment. On the contrary, L-NAA and PTIO inhibited the generation of NO in cells. MeJA treatment also led to a great increase in H2O2 level in suspension cells, but SNP treatment did not. In addition, PAL activities as well as MDA and phytoalexin contents displayed similar dynamic curves with different treatments, increasing firstly and then decreasing with peak activity or content at 4-6h. Those findings indicated that MeJA induced the generation of NO, and then MeJA and NO worked together to increase H2O2 level in suspension cells. Phytoalexin and MDA increase as well as PAL activity enhancement were mainly induced by NO, although it seemed other signals involved. |
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| Keywords: | PAL |
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