| 猪细小病毒单克隆抗体杂交瘤细胞株的建立及初步应用 |
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| 引用本文： | 陆苹,胡志贤,王国丰,周培,黄保青.猪细小病毒单克隆抗体杂交瘤细胞株的建立及初步应用[J].上海交通大学学报(农业科学版),1992(2). |
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| 作者姓名： | 陆苹 胡志贤 王国丰 周培 黄保青 |
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| 作者单位： | 上海农学院农业生物技术研究所 上海201101 |
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| 摘 要： | 用经超滤浓缩、PEG 沉淀,氯化铯密度梯度离心纯化的猪细小病毒(PPV)免疫 BALB/C 小白鼠,取免疫鼠脾细胞与骨髓瘤细胞 SP_2/O在PEG 存在下融合,经 HAT 筛选、ELlSA 测定及3次克隆化后,获得3株(P-15—36—6,P-15—45—51,P—15—52—28)能持续稳定分泌抗 PPV 单克隆抗体的杂交瘤细胞株,3株杂交瘤细胞的染色体数均在95条以上,经分类测定,其免疫球蛋白均属 Ig G,且均为 IgG_1亚类。阻断试验证明单抗具有抗 PPV 的特异性。利用间接 ELISA 抑制试验进行了单抗在诊断上应用研究。
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| 关 键 词： | 细小病毒 单克隆抗体 酶联免疫吸附试验 |
THE ESTABLISHMENT OF HYBRIDOMAS CELL LINES SECRETING MONOCLONAL ANTIBODIES TO PORCINE PARVOVIRUS(PPV) |
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| Lu Ping Hu Zixian Wang Guofeng Zhou Pei Huang Baoqing.THE ESTABLISHMENT OF HYBRIDOMAS CELL LINES SECRETING MONOCLONAL ANTIBODIES TO PORCINE PARVOVIRUS(PPV)[J].Journal of Shanghai Jiaotong University (Agricultural Science),1992(2). |
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| Authors: | Lu Ping Hu Zixian Wang Guofeng Zhou Pei Huang Baoqing |
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| Abstract: | BALB/C mice were immunized with porcine parvovirus which had been purified by ultrafiltering concentration,precipitation in PEG and ultracentrifugation in CsCl gra- dient.After HAT selection,cloning three times and ELISA test,three positive hybridoma cell lines(P-15-36-6,P-15-45-51,P-15-52-28)were established from the fusion of murine myeloma cells( SP_2/O)and spleen cells of BALB/C immunized with PPV.Their chromosome are more than 95 and their immunoglobulin class/subclass are all IgG_t.Ac- cording to bloking test,we recognized that the McAb secrected by the cell lines No. P-15-45-15 show a speciality to PPV.It has been investigated that the monoclonal antibody was applied in detection of PPV by the indirect ELISA inhibiting assay. |
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| Keywords: | Porcine parvovirus McAb ELISA |
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