| 节杆菌AD26的分离鉴定及其与假单胞菌ADP对阿特拉津的联合降解 |
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| 引用本文： | 朱希坤,李清艳,蔡宝立.节杆菌AD26的分离鉴定及其与假单胞菌ADP对阿特拉津的联合降解[J].农业环境科学学报,2009,28(3). |
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| 作者姓名： | 朱希坤 李清艳 蔡宝立 |
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| 作者单位： | 南开大学微生物学系和生物活性材料教育部重点实验室,天津,300071 |
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| 摘 要： | 用富集培养法,从农药厂的工业废水中分离到高效降解除草剂阿特拉津的AD26菌株,通过16S rRNA基因序列分析,该菌株被鉴定为节杆菌(A rthrobacter sp.).降解基因的PCR分析表明,AD26含有阿特拉津降解基因trzN和atzBC,它能以阿特拉津为唯一氮源、蔗糖或柠檬酸钠为碳源生长,将阿特拉津降解成氰尿酸,降解速度快但降解不完全.假单胞菌(Pseudomonas sp.)ADP是Waekea实验室分离的阿特拉津降解菌株,含有阿特拉津降解基因atzABCDEF,能以阿特拉津为唯一氮源、柠檬酸钠为碳源(不能以蔗糖为碳源)生长.将阿特拉津降解成NH3,和CO2,降解完全但降解速度慢.在阿特拉津浓度为200 mg·L-1的无机盐培养基中进行的AD26和ADP混合培养表明,它们对阿特拉津的降解发生了互补和增强作用,两个菌株能在以阿特拉津为唯一氮源、蔗糖为碳源的培养基中生长,而且生长和降解速率都好于单个菌株,培养72 h后阿特拉津去除率达到99.9%,其中76.7%的阿特拉津被降解成NH3和CO2.这表明由节杆菌AD26和假单胞菌ADP组成的混合菌株在阿特拉津废水处理和污染土壤的生物修复中有很好的应用潜力.
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| 关 键 词： | 节杆菌AD26 假单胞菌ADP 阿特拉津 联合降解 |
Isolation and Identification of Arthrobacter sp.AD26 and Joint Degradation of Atrazine by Arthrobacter sp.AD26 and Pseudomonas sp.ADP |
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| ZHU Xi-kun,LI Qing-yan,CAI Bao-li.Isolation and Identification of Arthrobacter sp.AD26 and Joint Degradation of Atrazine by Arthrobacter sp.AD26 and Pseudomonas sp.ADP[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2009,28(3). |
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| Authors: | ZHU Xi-kun LI Qing-yan CAI Bao-li |
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| Institution: | Department of Microbiology;and Key Laboratory of Bioactive Materials;Ministry of Education;Nankai University;Tianjin 300071;China |
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| Abstract: | A highly efficient atrazine-degrading bacterium, strain AD26, was isolated from an industrial wastewater sample using enrichment culture.16S rRNA gene sequencing identified AD26 as an Arthrobacter sp.strain.PCR assays for degradation genes indicated that AD26 contains atrazine-degrading genes trzN and atzBC.This strain uses atrazine as sole source of nitrogen and sucrose or sodium citrate as the carbon source for growth and degrades atrazine to cyanuric acid.Although AD26 exhibited a higher degradation rate... |
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| Keywords: | Arthrobacter sp AD26 Pseudomonas sp ADP atrazine joint degradation |
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