| GnRH-6与麦芽糖结合蛋白的融合表达及鉴定 |
| |
| 引用本文： | 杨亚萍,方富贵,章孝荣.GnRH-6与麦芽糖结合蛋白的融合表达及鉴定[J].动物医学进展,2007,28(7):12-16. |
| |
| 作者姓名： | 杨亚萍 方富贵 章孝荣 |
| |
| 作者单位： | 安徽农业大学动物科技学院,安徽合肥,230036 |
| |
| 基金项目： | 安徽省教育厅自然科学基金 |
| |
| 摘 要： | 将人工合成的哺乳动物型GnRH六聚体基因插入原核表达质粒pMAL-c2中,重组质粒转化到大肠埃希菌BL21中,采用IPTG进行诱导表达,用多糖树脂亲和层析法纯化蛋白,用SDS-PAGE对纯化蛋白进行分析,用间接ELISA方法鉴定融合蛋白的反应原性.结果表明,与麦芽糖结合蛋白基因融合的GnRH六聚体基因在大肠埃希菌BL21中能够高效表达;用多糖树脂进行亲和层析纯化蛋白,具有良好的纯化效果;表达的融合蛋白分子质量大小与理论值一致,且融合蛋白与GnRH抗体的反应较好.
|
| 关 键 词： | GnRH六聚体 pMAl-c2x质粒 诱导表达 蛋白纯化 |
| 文章编号： | 1007-5038（2007）07-0012-05 |
| 修稿时间： | 2007-04-16 |
Expression and Identification of the Fusion of GnRH-6 with the Gene Coding for Maltose Binding Protein |
| |
| YANG Ya-ping,FANG Fu-gui,ZHANG Xiao-rong.Expression and Identification of the Fusion of GnRH-6 with the Gene Coding for Maltose Binding Protein[J].Progress In Veterinary Medicine,2007,28(7):12-16. |
| |
| Authors: | YANG Ya-ping FANG Fu-gui ZHANG Xiao-rong |
| |
| Institution: | Animal Technological College ,Anhui Agricultural University, He f ei ,Anhui , 230036, China |
| |
| Abstract: | The synthetic mammalian GnRH-6 gene was inserted into the expression plasmid pMAL-c2. The recombinant plasmid was transformed into E. coli BL21, where the expression of GnRH-6 gene was induced with IPTG. Amylose resin was used to purify the protein by the affinity chromatograph method. The purified products were analyzed using the SDS-PAGE. The reaetiongenieity of fusion protein was identified by indirect ELISA. The results showed that the GnRH-6 gene in fusion with the gene encoding maltose binding protein could be expressed at a high level. The fusion protein could be purified efficiently by affinity chromatograph method. The molecular weight of the fusion protein was equal to that of the theory. the reaetiongenieity of fusion protein with GnRH antibody was very high. |
| |
| Keywords: | GnRH-6 expression plasmid pMAL-e2 induced expression purification of protein |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
