| EGF和IGF-1对水牛卵母细胞体外成熟培养的影响 |
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| 引用本文： | 王晓丽,农垚峰,冯贵雪,石德顺,韦精卫,周红英.EGF和IGF-1对水牛卵母细胞体外成熟培养的影响[J].中国畜牧兽医,2007,34(5):57-59. |
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| 作者姓名： | 王晓丽 农垚峰 冯贵雪 石德顺 韦精卫 周红英 |
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| 作者单位： | 广西大学动物科学技术学院，南宁 530005 |
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| 摘 要： | 作者研究了在培养液中添加不同浓度的EGF、IGF-1以及EGF联合IGF-1对水牛卵母细胞体外成熟的影响。结果表明：①添加各种浓度的EGF都可以提高水牛卵母细胞的成熟率，其中50 ng/ml EGF有显著影响(P＜0.05)。②10、20 ng/ml的IGF 1对水牛卵母细胞体外成熟无显著影响(P＞0.05)； 30 ng/ml的IGF-1能显著提高水牛卵母细胞体外成熟率(P＜0.05)。③添加20 ng/ml EGF+30 ng/ml IGF-1组卵母细胞体外成熟率高于添加30 ng/ml的IGF-1组，显著高于添加20 ng/ml EGF组和对照组(P＜0.05)。可见，EGF和IGF-1对水牛卵母细胞体外成熟有协同作用。
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| 关 键 词： | EGF IGF-1 卵母细胞 体外成熟 |
| 文章编号： | 1671-7236（2007）05-0057-03 |
| 修稿时间： | 2006-11-06 |
Effects of EGF and IGF-1 on Maturation of Buffalo Oocytes in Vitro |
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| WANG Xiao-li,NONG Yao-feng,FENG Gui-xue,SHI De-shun,WEI Jing-wei,ZHOU Hong-ying.Effects of EGF and IGF-1 on Maturation of Buffalo Oocytes in Vitro[J].China Animal Husbandry & Veterinary Medicine,2007,34(5):57-59. |
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| Authors: | WANG Xiao-li NONG Yao-feng FENG Gui-xue SHI De-shun WEI Jing-wei ZHOU Hong-ying |
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| Institution: | College of Animal Science and Technology, Guangxi University, Nanning 530005,China |
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| Abstract: | Three experiments were conducted to determine the effect of EGF and IGF 1 on maturation of buffalo oocytes in vitro. Oocytes were cultured in oocytes maturation medium with EGF and IGF-1 alone or combinedly at different concentration. The results in experimentⅠshowed that there were significant different between control group and the group with EGF(10，20，30，50 ng/ml) in maturation rate, the group with 50 ng/ml EGF was significantly higher than others(P＜0.05). In experiment II the maturation rate of buffalo oocytes was improved significantly in the groups with IGF(30 ng/ml)(P＜0.05), but not in the group with 10 ng/ml IGF-1. In experiment III the maturation rate in the group containing 20 ng/ml EGF and 30 ng/ml IGF-1 was significantly higher than 30 ng/ml IGF-1 or 20 ng/ml EGF groups(P＜0.05). The results obtained in this study indicate that EGF and IGF-1 have coeffects on maturation of buffalo oocytes in vitro. |
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| Keywords: | EGF IGF-1 |
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